D'Aléo Anthony, Pompidor Guillaume, Elena Bénédicte, Vicat Jean, Baldeck Patrice L, Toupet Loïc, Kahn Richard, Andraud Chantal, Maury Olivier
Laboratoire de Chimie, ENS-Lyon, CNRS, 46 Allée d'Italie, 69364 Lyon, France.
Chemphyschem. 2007 Oct 8;8(14):2125-32. doi: 10.1002/cphc.200700375.
The linear and non-linear photophysical properties of tris-dipicolinate europium and terbium complexes (absorption, emission, lifetime, luminescence induced by two-photon absorption) are studied in the crystalline state as well as in protein derivative crystals and compared to those in solution. Upon laser irradiation at 532 nm, luminescence of terbium is induced by a two-photon antenna effect, whereas luminescence of europium results from one-photon absorption in forbidden f-f transitions. Finally, linear and two-photon microscopy imaging experiments on biological and bio-inspired crystals are performed. These first proof-of-concept experiments open the way for the development of time-resolved non-linear microscopy that should combine the advantages of lanthanide luminescence (long lifetime, sharp emission bands, insensitivity to oxygen) with those of confocal biphotonic excitation (near-IR excitation, 3D resolution and reduced photodamage).
研究了三吡啶甲酸铕和铽配合物在晶体状态以及蛋白质衍生物晶体中的线性和非线性光物理性质（吸收、发射、寿命、双光子吸收诱导的发光），并与溶液中的性质进行了比较。在532nm激光照射下，铽的发光是由双光子天线效应诱导的，而铕的发光则来自于禁戒f-f跃迁中的单光子吸收。最后，对生物和仿生晶体进行了线性和双光子显微镜成像实验。这些首次概念验证实验为时间分辨非线性显微镜的发展开辟了道路，该显微镜应结合镧系发光的优点（长寿命、尖锐发射带、对氧不敏感）和共焦双光子激发的优点（近红外激发、三维分辨率和减少光损伤）。
