Krushel L A, Connolly J A, van der Kooy D
Department of Anatomy, University of Toronto, Ont., Canada.
Brain Res Dev Brain Res. 1989 May 1;47(1):137-42. doi: 10.1016/0165-3806(89)90116-8.
Mechanisms involved in the developmental organization of the rat striatum were investigated in vitro. The neurons of the patch and matrix compartments were preferentially labeled in vivo with a [3H]thymidine injection on embryonic day (E) 13 or 18, respectively. Two or 7 days later the striatum was removed, dissociated into a single cell suspension and plated on a collagen-coated substrate. After 5 days in culture the neurons had migrated into aggregates. Within an individual aggregate, neurons labeled on E13 tended to clump together, whereas neurons labeled on E18 were randomly dispersed. Comparing between aggregates, [3H]thymidine-labeled E13 cells were located in aggregates containing numerous other labeled E13 cells, whereas [3H]thymidine-labeled E18 cells were dispersed randomly between aggregates. These results suggest that early born striatal neurons (primarily patch cells) selectively associate with each other, and that this process may be crucial to the developmental compartmentalization of the rat striatum.
在体外研究了大鼠纹状体发育组织中的机制。分别在胚胎第（E）13天或18天通过注射[3H]胸腺嘧啶核苷在体内对斑块和基质区室的神经元进行优先标记。2天或7天后取出纹状体，解离成单细胞悬液并接种在胶原包被的基质上。培养5天后，神经元迁移形成聚集体。在单个聚集体内，E13标记的神经元倾向于聚集在一起，而E18标记的神经元则随机分散。在聚集体之间进行比较时，[3H]胸腺嘧啶核苷标记的E13细胞位于含有许多其他标记的E13细胞的聚集体中，而[3H]胸腺嘧啶核苷标记的E18细胞则随机分散在聚集体之间。这些结果表明，早期生成的纹状体神经元（主要是斑块细胞）彼此选择性地结合，并且这个过程可能对大鼠纹状体的发育分区至关重要。
