Graybiel A M, Hickey T L
Proc Natl Acad Sci U S A. 1982 Jan;79(1):198-202. doi: 10.1073/pnas.79.1.198.
Neurons being generated in the striatum of 10 fetal cats were pulse labeled by injection of [3H]thymidine directly into the maternal uterus at times ranging between the 22nd and 30th days (E22-E30) of the 65-day gestational period. Many of the striatal neurons labeled during this interval were found, at adolescence, to form 100- to 600-mum-wide cell clusters in the caudate nucleus. In E24-E30 specimens, we compared the distributions of these cell clusters with the locations of patches of low acetylcholinesterase activity and high enkephalin immunoreactivity (the "striosomes') visualized in serially adjoining sections. We found precise matches between most of the cell clusters and the acetylcholinesterase-poor enkephalin-rich zones, regardless of the embryonic age at which exposure to the [3H]thymidine had occurred. We conclude that the histochemically distinct striosomal patch-works observed in the acetylcholinesterase and enkephalin preparations correspond to ontogenetic units of the striatum.
在65天妊娠期的第22天至第30天（E22 - E30）期间，通过将[3H]胸腺嘧啶核苷直接注入母体子宫，对10只胎猫纹状体中正在生成的神经元进行脉冲标记。在这个时间段内标记的许多纹状体神经元，在青春期时，在尾状核中形成了宽度为100至600微米的细胞簇。在E24 - E30标本中，我们将这些细胞簇的分布与在连续相邻切片中可视化的低乙酰胆碱酯酶活性和高脑啡肽免疫反应性斑块（“纹状体小体”）的位置进行了比较。我们发现，无论接触[3H]胸腺嘧啶核苷时的胚胎年龄如何，大多数细胞簇与乙酰胆碱酯酶含量低、脑啡肽含量高的区域精确匹配。我们得出结论，在乙酰胆碱酯酶和脑啡肽制剂中观察到的组织化学上不同的纹状体小体网络对应于纹状体的个体发生单位。
