Key Laboratory of Biofuels, Key Laboratory of Shandong Energy Biological Genetic Resources, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China.
University of Chinese Academy of Sciences, Beijing 100049, China.
Int J Mol Sci. 2019 Sep 5;20(18):4356. doi: 10.3390/ijms20184356.
Palmitoleic acid, one scarce omega-7 monounsaturated fatty acid, has important applications in the fields of medicine and health products. has been considered as a promising candidate for the production of palmitoleic acid due to its high lipid and palmitoleic acid content and remarkable heterotrophic ability. The high-density heterotrophic cultivation of was conducted in this work, and the highest biomass of 42.9 g L and a relatively low lipid content of 28.7% were observed. To further enhance the lipid and palmitoleic acid accumulation, induction strategies under two regimes of phototrophy and heterotrophy with different conditions were investigated and compared. Results demonstrated encouraging promotions both by heterotrophic and phototrophic ways, and the final lipid contents reached 41.9% and 49.0%, respectively. In consideration of the time cost, however, the induction under heterotrophic conditions was much more advantageous, by which the highest lipid and palmitoleic acid productivities of 1.77 g L d and 924 mg L d were obtained respectively, with the lipid yield on glucose of 0.26 g g.
棕榈油酸是一种罕见的ω-7 单不饱和脂肪酸，在医药和保健品领域有重要的应用。由于其脂质和棕榈油酸含量高，异养能力显著，已被认为是生产棕榈油酸的有前途的候选物。本工作进行了高密度异养培养，观察到最高生物量为 42.9 g/L，相对较低的脂质含量为 28.7%。为了进一步提高脂质和棕榈油酸的积累，研究并比较了两种光照和异养条件下的诱导策略。结果表明，通过异养和自养两种方式都有令人鼓舞的促进作用，最终的脂质含量分别达到 41.9%和 49.0%。然而，考虑到时间成本，异养条件下的诱导更具优势，通过该方法获得了最高的脂质和棕榈油酸生产力，分别为 1.77 g/L/d 和 924 mg/L/d，葡萄糖的脂质产率为 0.26 g/g。
