Noble B, Du Bois R M, Poulter L W
Department of Immunology, Royal Free Hospial, London, UK.
Clin Exp Immunol. 1989 Apr;76(1):41-6.
Monoclonal antibodies that identify phenotypically distinct macrophage subsets were used to analyse the macrophages in lung biopsy specimens and bronchoalveolar lavage fluid from patients with cryptogenic fibrosing alveolitis. Among the antibodies were RFD1, an interdigitating cell marker, RFD7, a marker for mature tissue macrophages, and RFD9, which identifies epithelioid cells as well as germinal centre macrophages. The lavage fluid was found to contain abnormally high numbers of cells staining with each of the antibodies, a finding that could be explained, at least in part, by an increased frequency of cells expressing more than one marker. In lung tissue macrophage phenotypes within the interstitium were found to differ significantly from those in the alveolar space. Most strikingly, cells bearing the antigen recognized by RFD9 were entirely absent from the interstitial macrophage population, whereas the vast majority in the alveolar lumen were RFD9-positive. The discrete compartmentalization of phenotypically different macrophages within the lung suggests that macrophages may contribute differently to lung pathology in each microenvironment. The functional capacity of the unusual RFD9-positive alveolar macrophages remains to be determined, but their close association with the process of alveolar occlusion indicates a role in the fibrotic process.
利用能识别表型不同的巨噬细胞亚群的单克隆抗体，对隐源性纤维化肺泡炎患者肺活检标本及支气管肺泡灌洗液中的巨噬细胞进行分析。这些抗体包括RFD1（一种树突状细胞标志物）、RFD7（成熟组织巨噬细胞的标志物）以及RFD9（可识别上皮样细胞和生发中心巨噬细胞）。发现灌洗液中用每种抗体染色的细胞数量异常高，这一发现至少部分可以通过表达不止一种标志物的细胞频率增加来解释。在肺组织中，间质内的巨噬细胞表型与肺泡腔内的巨噬细胞表型存在显著差异。最显著的是，间质巨噬细胞群体中完全不存在携带RFD9识别抗原的细胞，而肺泡腔内绝大多数细胞是RFD9阳性的。肺内表型不同的巨噬细胞的离散分隔表明，巨噬细胞在每个微环境中对肺部病理的贡献可能不同。异常的RFD9阳性肺泡巨噬细胞的功能能力尚待确定，但其与肺泡闭塞过程的密切关联表明其在纤维化过程中发挥作用。
