Campbell D A, Poulter L W, Janossy G, du Bois R M
Thorax. 1985 Jun;40(6):405-11. doi: 10.1136/thx.40.6.405.
Immunohistological analysis using monoclonal antibodies in conjunction with histochemical techniques has been applied to lung biopsy material from patients with cryptogenic fibrosing alveolitis. Subsets of lymphoid and non-lymphoid cells have been identified in situ. This analysis showed that the inflammatory cells present were predominantly mononuclear. Most of the lymphoid cells were B lymphocytes, organised into follicles with occasional germinal centre formation. IgM was the major class of immunoglobulin expressed. Both T4+ and T8+ lymphocytes were seen diffusely distributed in the interstitium. The T4+ positive cells were also seen within the B lymphoid follicles. Almost all non-lymphoid cells expressed the phenotype of inflammatory macrophages, but a few also expressed a phenotype characteristic of interdigitating cells. These results suggest that a local B lymphoid immune response is occurring in cryptogenic fibrosing alveolitis. The possibility that a cell mediated immune response is also emerging is discussed.
运用单克隆抗体结合组织化学技术的免疫组织学分析已应用于隐源性纤维性肺泡炎患者的肺活检材料。已在原位鉴定出淋巴细胞和非淋巴细胞亚群。该分析表明，存在的炎性细胞主要为单核细胞。大多数淋巴细胞是B淋巴细胞，组织成滤泡，偶尔形成生发中心。IgM是表达的主要免疫球蛋白类别。T4 +和T8 +淋巴细胞均可见弥漫分布于间质中。T4 +阳性细胞也可见于B淋巴滤泡内。几乎所有非淋巴细胞都表达炎性巨噬细胞的表型，但也有少数表达指状突细胞的特征性表型。这些结果表明，隐源性纤维性肺泡炎中正在发生局部B淋巴细胞免疫反应。文中还讨论了细胞介导的免疫反应也正在出现的可能性。
