胎儿舌器官培养中味蕾的非神经依赖性和异位额外诱导
Nerve-independent and ectopically additional induction of taste buds in organ culture of fetal tongues.
作者信息
Honda Kotaro, Tomooka Yasuhiro
机构信息
Graduate School of Industrial Science and Technology, Department of Biological Science and Technology, Tokyo University of Science, 6-3-1 Niijuku, Katsushika-ku, Tokyo, 125-8585, Japan.
出版信息
In Vitro Cell Dev Biol Anim. 2016 Oct;52(9):911-919. doi: 10.1007/s11626-016-0067-4. Epub 2016 Jul 1.
An improved organ culture system allowed to observe morphogenesis of mouse lingual papillae and taste buds relatively for longer period, in which fetal tongues were analyzed for 6 d. Taste cells were defined as eosinophobic epithelial cells expressing CK8 and Sox2 within lingual epithelium. Addition of glycogen synthase kinase 3 beta inhibitor CHIR99021 induced many taste cells and buds in non-gustatory and gustatory stratified lingual epithelium. The present study clearly demonstrated induction of taste cells and buds ectopically and without innervation.
一种改良的器官培养系统能够相对较长时间地观察小鼠舌乳头和味蕾的形态发生，在此系统中对胎鼠舌进行了6天的分析。味觉细胞被定义为舌上皮内表达细胞角蛋白8（CK8）和性别决定区Y框蛋白2（Sox2）的嗜伊红性上皮细胞。添加糖原合酶激酶3β抑制剂CHIR99021可在非味觉和味觉分层舌上皮中诱导产生许多味觉细胞和味蕾。本研究清楚地证明了在无神经支配的情况下异位诱导产生味觉细胞和味蕾。
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