Department of Biological Science and Technology and Research Center for RNA Science, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan.
In Vitro Cell Dev Biol Anim. 2011 Apr;47(4):333-40. doi: 10.1007/s11626-011-9398-3. Epub 2011 Mar 25.
A taste bud is a sensory organ and consists of 50-100 spindle-shaped cells. The cells function as taste acceptors. They have characteristics of both epithelial and neuronal cells. A taste bud contains four types of cells, type I, type II, type III cells, and basal cells. Taste buds were isolated from a tongue of a p53-deficient mouse at day 12, and 11 clonal taste bud (TBD) cell lines were established. In immunochemical analysis, all cell lines expressed cytokeratin 18, gustducin, T1R3, and neural cellular adhesion molecule, but not GLAST. In RT-PCR analysis, shh was not expressed in any of the cell lines. Further analysis with RT-PCR was conducted on four cell lines. They expressed G protein-coupled taste receptors; T1R3, T2R8 for sweet, bitter, umami. And they also expressed α-ENaC for salty taste. While, a candidate for sour receptor HCN4 was expressed in TBD-a1 and TBD-a7 lines. And another candidate for sour receptor PKD1L3 was slightly expressed in TBD-a1 and TBD-c1.
味蕾是一种感觉器官，由 50-100 个梭形细胞组成。这些细胞作为味觉感受器发挥作用。它们具有上皮细胞和神经元细胞的特征。一个味蕾包含四种类型的细胞，即 I 型、II 型、III 型细胞和基底细胞。从第 12 天缺乏 p53 的老鼠舌头上分离出味蕾，并建立了 11 个克隆的味蕾（TBD）细胞系。在免疫化学分析中，所有细胞系均表达细胞角蛋白 18、味觉素、T1R3 和神经细胞黏附分子，但不表达 GLAST。在 RT-PCR 分析中，没有任何细胞系表达 shh。进一步对四个细胞系进行 RT-PCR 分析。它们表达了与味觉相关的 G 蛋白偶联受体；T1R3、T2R8 可感知甜、苦、鲜味。它们还表达了用于感知咸味的 α-ENaC。而候选酸受体 HCN4 在 TBD-a1 和 TBD-a7 系中表达，候选酸受体 PKD1L3 在 TBD-a1 和 TBD-c1 系中则有微弱表达。
