Liu Fengqiao, Su Baofeng, Gao Chengbin, Zhou Shun, Song Lin, Tan Fenghua, Dong Xiaoyu, Ren Yichao, Li Chao
Marine Science and Engineering College, Qingdao Agricultural University, Qingdao, 266109, China.
Ministry of Agriculture Key Laboratory of Freshwater Aquatic Biotechnology and Breeding, Heilongjiang Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin, 150070, China; National and Local Joint Engineering Laboratory of Freshwater Fish Breeding, Heilongjiang Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin, 150070, China.
Fish Shellfish Immunol. 2016 Aug;55:654-61. doi: 10.1016/j.fsi.2016.06.047. Epub 2016 Jun 28.
The pathogen recognition receptors (PRRs), which can recognize the conserved pathogen-associated molecular patterns (PAMPs) of the bacteria, play key roles in the mucosal surfaces for pathogen recognition and activation of immune signaling pathways. However, our understanding of the PRRs and their activities in mucosal surfaces in the critical early time points during pathogen infection is still limited. Towards to this end, here, we sought to identify the Toll-like receptor 2 (TLR2) in turbot as well as its expression profiles in mucosal barriers following bacterial infection in the early time points. The full-length TLR2 transcript consists of open reading frame (ORF) of 2451 bp encoding the putative peptide of 816 amino acids. The phylogenetic analysis revealed the turbot TLR2 showed the closest relationship to Paralichthys olivaceus. The TLR2 mRNA expression could be detected in all examined tissues, with the most abundant expression level in liver, and the lowest expression level in skin. In addition, TLR2 showed different expression patterns following Vibrio anguillarum and Streptococcus iniae infection, but was up-regulated following both challenge, especially post S. iniae challenge. Characterization of TLR2 will probably contribute to understanding of a number of infectious diseases and broaden the knowledge of interactions between host and pathogen, which will eventually help in the development of novel intervention strategies for farming turbot.
病原体识别受体（PRRs）能够识别细菌保守的病原体相关分子模式（PAMPs），在黏膜表面的病原体识别和免疫信号通路激活中发挥关键作用。然而，我们对病原体感染关键早期阶段黏膜表面PRRs及其活性的了解仍然有限。为此，我们试图鉴定大菱鲆中的Toll样受体2（TLR2）及其在细菌感染早期黏膜屏障中的表达谱。TLR2转录本全长包含2451 bp的开放阅读框（ORF），编码816个氨基酸的假定肽。系统发育分析表明，大菱鲆TLR2与褐牙鲆的关系最为密切。在所有检测组织中均可检测到TLR2 mRNA表达，肝脏中表达水平最高，皮肤中表达水平最低。此外，TLR2在鳗弧菌和海豚链球菌感染后呈现不同的表达模式，但在两种菌感染后均上调，尤其是在海豚链球菌感染后。TLR2的特性研究可能有助于理解多种传染病，并拓宽宿主与病原体相互作用的知识，最终有助于开发养殖大菱鲆的新型干预策略。
