Gao Chengbin, Fu Qiang, Su Baofeng, Song Huanhuan, Zhou Shun, Tan Fenghua, Li Chao
Marine Science and Engineering College, Qingdao Agricultural University, Qingdao 266109, China.
Marine Science and Engineering College, Qingdao Agricultural University, Qingdao 266109, China; State Key Laboratory of Estuarine and Coastal Research, East China Normal University, Shanghai 200062, China.
Fish Shellfish Immunol. 2017 Jul;66:270-279. doi: 10.1016/j.fsi.2017.05.030. Epub 2017 May 10.
Cathepsin F (CTSF) is a recently described papain-like cysteine protease and unique among cathepsins due to an elongated N-terminal pro-region, which contains a cystatin domain. CTSF likely plays a regulatory role in processing the invariant chain which is associated with the major histocompatibility complex (MHC) class II. In this regard, we identified the CTSF gene of turbot as well as its protein structure, phylogenetic relationships, and expression patterns in mucosal tissues following Vibrio anguillarum and Streptococcus iniae challenge. We also determined the expression patterns of CTSF in mucosal tissues after vaccinated with the formalin-inactivated V. vulnificus whole-cell vaccine. Briefly, turbot CTSF gene showed the closest relationship with that of Paralichthys olivaceus in phylogenetic analysis. And CTSF was ubiquitously expressed in all tested tissues with the highest expression level in gill. In addition, CTSF gene showed different expression patterns following different bacterial challenge. The significant quick regulation of CTSF in mucosal surfaces against infection indicated its roles in mucosal immunity. Functional studies should further characterize avail utilization of CTSF function to increase the disease resistance of turbot in maintaining the integrity of the mucosal barriers against infection and to facilitate selection of the disease resistant family/strain in turbot.
组织蛋白酶F（CTSF）是一种最近被描述的木瓜蛋白酶样半胱氨酸蛋白酶，由于其N端前肽区域较长，其中包含一个胱抑素结构域，因此在组织蛋白酶中具有独特性。CTSF可能在处理与主要组织相容性复合体（MHC）II类相关的恒定链中发挥调节作用。在这方面，我们鉴定了大菱鲆的CTSF基因及其蛋白质结构、系统发育关系，以及鳗弧菌和海豚链球菌攻击后黏膜组织中的表达模式。我们还测定了用福尔马林灭活的创伤弧菌全细胞疫苗接种后CTSF在黏膜组织中的表达模式。简而言之，在系统发育分析中，大菱鲆CTSF基因与褐牙鲆的CTSF基因关系最为密切。CTSF在所有测试组织中均有广泛表达，在鳃中的表达水平最高。此外，CTSF基因在不同细菌攻击后表现出不同的表达模式。CTSF在黏膜表面对感染的显著快速调节表明其在黏膜免疫中的作用。功能研究应进一步表征CTSF功能的有效利用，以提高大菱鲆在维持黏膜屏障抗感染完整性方面的抗病能力，并有助于选择大菱鲆抗病家族/品系。
