Li Chao, Gao Chengbin, Fu Qiang, Su Baofeng, Chen Jinghua
Marine Science and Engineering College, Qingdao Agricultural University, Qingdao 266109, China.
National and Local Joint Engineering Laboratory of Freshwater Fish Breeding, Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China; Ministry of Agriculture Key Laboratory of Freshwater Aquatic Biotechnology and Breeding, Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China.
Fish Shellfish Immunol. 2017 Sep;68:386-394. doi: 10.1016/j.fsi.2017.07.032. Epub 2017 Jul 18.
Fetuin B (FETUB), a recently described cysteine proteinase inhibitor, has numerous conserved N-glycosylation sites, species-specific O-glycosylation sites, and two cystatin (CY) domains. FETUB is likely to play regulatory roles in acute inflammation, female infertility, fish organogenesis and tumor suppression. In the present study, transcript of turbot FETUB gene was captured, its protein structure and expression patterns in different tissues with emphasis on mucosal barriers following different bacterial infection were characterized. Turbot FETUB gene showed the closest relationship with Takifugu rubripes in phylogenetic analysis. In addition, FETUB was ubiquitously expressed in all examined tissues with the highest expression level in skin. Finally, FETUB gene showed different expression patterns following both bacterial challenge. The rapidly and significantly differential expression patterns of FETUB in mucosal surfaces against bacterial infections might indicate its key roles to prevent pathogen attachment and entry in turbot mucosal immunity. Functional studies should be carried out to further characterize the FETUB and avail utilization of its function to increase the disease resistance of turbot in maintaining the integrity of the mucosal barriers against infections and to facilitate selection of the fine family/varieties of disease resistance in turbot.
胎球蛋白B（FETUB）是一种最近被描述的半胱氨酸蛋白酶抑制剂，具有众多保守的N-糖基化位点、物种特异性O-糖基化位点以及两个胱抑素（CY）结构域。FETUB可能在急性炎症、女性不孕、鱼类器官发生和肿瘤抑制中发挥调节作用。在本研究中，捕获了大菱鲆FETUB基因的转录本，对其蛋白质结构以及在不同组织中的表达模式进行了表征，重点研究了不同细菌感染后黏膜屏障的情况。在系统发育分析中，大菱鲆FETUB基因与红鳍东方鲀的关系最为密切。此外，FETUB在所有检测组织中均有广泛表达，在皮肤中的表达水平最高。最后，在两种细菌攻击后，FETUB基因呈现出不同的表达模式。FETUB在黏膜表面针对细菌感染的快速且显著的差异表达模式可能表明其在大菱鲆黏膜免疫中防止病原体附着和侵入方面的关键作用。应开展功能研究以进一步表征FETUB，并利用其功能提高大菱鲆的抗病能力，维持黏膜屏障抵御感染的完整性，促进大菱鲆抗病优良家系/品种的选育。
