黏膜相关恒定T细胞在强直性脊柱炎中的作用

Involvement of Mucosal-associated Invariant T cells in Ankylosing Spondylitis.

作者信息

Hayashi Eri, Chiba Asako, Tada Kurisu, Haga Keiichi, Kitagaichi Mie, Nakajima Shihoko, Kusaoi Makio, Sekiya Fumio, Ogasawara Michihiro, Yamaji Ken, Tamura Naoto, Takasaki Yoshinari, Miyake Sachiko

机构信息

From the Department of Internal Medicine and Rheumatology, and Department of Immunology, and Department of Gastroenterology, Juntendo University School of Medicine, Tokyo, Japan.E. Hayashi, MD, Department of Internal Medicine and Rheumatology, and Department of Immunology, Juntendo University School of Medicine; A. Chiba, MD, PhD, Department of Immunology, Juntendo University School of Medicine; K. Tada, MD, PhD, Department of Internal Medicine and Rheumatology, Juntendo University School of Medicine; K. Haga, MD, PhD, Department of Gastroenterology, Juntendo University School of Medicine; M. Kitagaichi, MD, Department of Internal Medicine and Rheumatology, and Department of Immunology, Juntendo University School of Medicine; S. Nakajima, MD, Department of Internal Medicine and Rheumatology, and Department of Immunology, Juntendo University School of Medicine; M. Kusaoi, MD, PhD, Department of Internal Medicine and Rheumatology, Juntendo University School of Medicine; F. Sekiya, MD, PhD, Department of Internal Medicine and Rheumatology, Juntendo University School of Medicine; M. Ogasawara, MD, PhD, Department of Internal Medicine and Rheumatology, Juntendo University School of Medicine; K. Yamaji, MD, PhD, Department of Internal Medicine and Rheumatology, Juntendo University School of Medicine; N. Tamura, MD, PhD, Department of Internal Medicine and Rheumatology, Juntendo University School of Medicine; Y. Takasaki, MD, PhD, Department of Internal Medicine and Rheumatology, Juntendo University School of Medicine; S. Miyake, MD, PhD, Department of Immunology, Juntendo University School of Medicine.

出版信息

J Rheumatol. 2016 Sep;43(9):1695-703. doi: 10.3899/jrheum.151133. Epub 2016 Jul 1.

DOI:10.3899/jrheum.151133

PMID:27370879

Abstract

OBJECTIVE

Ankylosing spondylitis (AS) is characterized by chronic inflammation of the axial and peripheral joints and ligamentous attachments. Gut immunity is thought to be involved in AS, because a prominent coexistence of gut and joint inflammation has been observed in patients with AS. Mucosal-associated invariant T (MAIT) cells are preferentially located in the gut lamina propria and produce inflammatory cytokines such as interleukin 17 (IL-17) and tumor necrosis factor-α (TNF-α), which are therapeutic targets for AS. This study aimed to investigate the involvement of MAIT cells in AS.

METHODS

The frequency of MAIT cells and their cytokine production were determined in patients with AS and healthy controls (HC). The expression of a MAIT cell activation marker (CD69) was analyzed in patients with AS by using flow cytometry.

RESULTS

The frequency of MAIT cells in the peripheral blood was lower in patients with AS compared with HC. The levels of IL-17 produced by MAIT cells after activation were higher in patients with AS than in the HC. CD69 expression on MAIT cells correlated with the Ankylosing Spondylitis Disease Activity Score in patients with AS.

CONCLUSION

These results suggest the involvement of MAIT cells in the pathogenesis of AS.

摘要

目的

强直性脊柱炎（AS）的特征是中轴和外周关节以及韧带附着点的慢性炎症。肠道免疫被认为与AS有关，因为在AS患者中已观察到肠道和关节炎症显著共存。黏膜相关恒定T（MAIT）细胞优先位于肠道固有层，并产生炎性细胞因子，如白细胞介素17（IL-17）和肿瘤坏死因子-α（TNF-α），这些都是AS的治疗靶点。本研究旨在调查MAIT细胞在AS中的作用。