Suzuki Y, Nagae Y, Ishikawa T, Watanabe Y, Nagashima T, Matsukubo K, Shimizu H
Department of Public Health, Jikei University School of Medicine, Tokyo, Japan.
Environ Mol Mutagen. 1989;13(4):314-8. doi: 10.1002/em.2850130406.
The micronucleus test is used widely as an in vivo short-term assay for potential carcinogens. In the present study, results of the micronucleus test were affected by the rate of erythropoiesis in the bone marrow erythropoietin, a growth factor for the erythroblast, which was used to induce erythropoiesis. The highest frequency of micronucleated polychromatic erythrocytes (MPCE) and a dose-response relationship between erythropoietin doses and MPCE frequency were seen 30 hr after injection of 1,1-dimethylhydrazine (DMH) to mice administered 24 hr previously with erythropoietin. The effect of erythropoietin was maximal when erythropoietin was given 24 hr before DMH, indicating that accelerating the multiplication of erythroblasts will increase the frequency of micronuclei induced by mutagens. Induction of MPCE in the bone marrow by four other compounds--benzo(a)pyrene, 2-naphthylamine, mitomycin C, and vincristine--was also increased by pretreatment with erythropoietin.
微核试验作为一种体内潜在致癌物短期检测方法被广泛应用。在本研究中，微核试验结果受骨髓红细胞生成速率的影响，红细胞生成素是成红细胞的生长因子，用于诱导红细胞生成。在给预先接受红细胞生成素处理24小时的小鼠注射1,1 - 二甲基肼（DMH）30小时后，观察到微核多染红细胞（MPCE）的频率最高，且红细胞生成素剂量与MPCE频率之间存在剂量反应关系。当在DMH注射前24小时给予红细胞生成素时，其效果最佳，这表明加速成红细胞的增殖会增加诱变剂诱导的微核频率。另外四种化合物——苯并(a)芘、2 - 萘胺、丝裂霉素C和长春新碱——对骨髓MPCE的诱导作用也因红细胞生成素预处理而增强。
