Suzuki Y, Shimizu H, Nagae Y, Fukumoto M, Okonogi H, Kadokura M
Department of Public Health and Environmental Medicine, Jikei University School of Medicine, Tokyo, Japan.
Environ Mol Mutagen. 1993;22(2):101-6. doi: 10.1002/em.2850220208.
The micronucleus test is used widely as an in vivo short-term assay for potential carcinogens. In the present study, results of the micronucleus test were affected by cobalt dichloride pretreatment. Cobalt dichloride was used to induce erythropoietin, a growth factor for erythropoiesis. The increase in mutagen-induced micronucleus response following cobalt pretreatment, therefore, may have been due to a change in the rate of erythropoiesis. The greatest interaction between cobalt pretreatment and mutagen treatment for the induction of micronucleated polychromatic erythrocytes (MPCE) occurred when mice were injected with 1,1-dimethylhydrazine (DMH) 12-24 hr after pretreatment with cobalt dichloride and killed 30 hr later. Increased sensitivity of the micronucleus test was attributable to the administration of mutagen during the differentiation and multiplication of erythroblast, which is presumed to have been accelerated by pretreatment with cobalt dichloride. An increased induction of MPCE in the bone marrow by two chemicals--benzo(a)pyrene, 2-naphthylamine--was also observed following pretreatment with cobalt dichloride.
微核试验被广泛用作一种检测潜在致癌物的体内短期试验。在本研究中，微核试验的结果受到二氯化钴预处理的影响。二氯化钴用于诱导促红细胞生成素，这是一种红细胞生成的生长因子。因此，钴预处理后诱变剂诱导的微核反应增加可能是由于红细胞生成速率的变化。当小鼠在二氯化钴预处理后12 - 24小时注射1,1 - 二甲基肼（DMH）并在30小时后处死时，钴预处理和诱变剂处理之间对诱导微核多染红细胞（MPCE）的相互作用最为显著。微核试验敏感性的增加归因于在成红细胞分化和增殖过程中给予诱变剂，推测这一过程因二氯化钴预处理而加速。在用二氯化钴预处理后，还观察到两种化学物质——苯并(a)芘、2 - 萘胺——对骨髓中MPCE的诱导增加。
