腹腔液中Th17细胞百分比增加与子宫内膜异位症的严重程度相关。
Increased percentage of Th17 cells in peritoneal fluid is associated with severity of endometriosis.
作者信息
Gogacz Marek, Winkler Izabela, Bojarska-Junak Agnieszka, Tabarkiewicz Jacek, Semczuk Andrzej, Rechberger Tomasz, Adamiak Aneta
机构信息
Oncology Centre of the Lublin, Jaczewski Street 7, 20-090 Lublin, Poland.
Oncology Centre of the Lublin, Jaczewski Street 7, 20-090 Lublin, Poland; II Department of Gynecology, Lublin Medical University, Jaczewski Street 8, 20-954 Lublin, Poland.
出版信息
J Reprod Immunol. 2016 Sep;117:39-44. doi: 10.1016/j.jri.2016.04.289. Epub 2016 May 20.
AIM
Th17 cells are a newly discovered T helper lymphocyte subpopulation, producing interleukin IL-17. Th17 cells are present in blood and peritoneal fluid (PF) at different stages of endometriosis. We aim to establish their potential importance in the pathogenesis and clinical features of the disease.
METHODS
The percentage of Th17 cells among T helper lymphocytes was determined in the PF and peripheral blood (PB) of patients with endometriosis and in the control group by flow cytometry using monoclonal antibodies: anti-CD-4-FITC, anti-CD-3-PE/Cy5, and anti-IL-17A-PE.
RESULTS
Th17 percentage is increased in PF in comparison with PB in both endometriotic patients and in the control group. In severe endometriosis, the percentage of Th17 cells in PF was higher than with early (I/II stage) endometriosis. A positive correlation between the percentage of Th17 cells in PF and the white blood cell count in PB was found in patients with endometriosis.
CONCLUSION
Targeting the activity of PF Th17 cells may have an influence on the proliferation of ectopic tissue and clinical manifestations of the disease.
目的
Th17细胞是新发现的产生白细胞介素IL - 17的辅助性T淋巴细胞亚群。在子宫内膜异位症的不同阶段，血液和腹腔液（PF）中均存在Th17细胞。我们旨在确定它们在该疾病发病机制和临床特征中的潜在重要性。
方法
采用单克隆抗体：抗CD - 4 - FITC、抗CD - 3 - PE/Cy5和抗IL - 17A - PE，通过流式细胞术测定子宫内膜异位症患者及对照组的PF和外周血（PB）中辅助性T淋巴细胞中Th17细胞的百分比。
结果
在子宫内膜异位症患者和对照组中，PF中的Th17百分比均高于PB。在重度子宫内膜异位症中，PF中Th17细胞的百分比高于早期（I/II期）子宫内膜异位症。在子宫内膜异位症患者中，PF中Th17细胞百分比与PB中白细胞计数之间存在正相关。
结论
针对PF中Th17细胞的活性可能会对异位组织的增殖和疾病的临床表现产生影响。
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