Oosterlynck D J, Meuleman C, Lacquet F A, Waer M, Koninckx P R
Department of Obstetrics and Gynecology, University of Leuven, Belgium.
Am J Reprod Immunol. 1994 Jan;31(1):25-31. doi: 10.1111/j.1600-0897.1994.tb00843.x.
We investigated the lymphocyte subpopulations in peripheral blood (PB) and peritoneal fluid (PF) of women with and without endometriosis to evaluate if the decreased natural killer (NK)-mediated cytotoxicity in women with endometriosis was due to a quantitative defect or not.
The PB and PF mononuclear cells of 59 women undergoing a diagnostic laparoscopy for pain and/or infertility were analyzed by flow cytometry.
The number and concentration of PF mononuclear cells (MC) was increased in women with endometriosis compared to women without endometriosis. The monocyte/macrophage marker (CD14) was expressed on 70.3 and 66.9% of PFMC of women with and without endometriosis, respectively. The CD4/CD8 ratio was inverted in the PF, and this was more pronounced in women with endometriosis. In the PF of women with endometriosis, 41.3% of the lymphocytes were CD8 positive, compared to 34.3% in women without endometriosis. The percentage of NK positive lymphocytes in PF, using three different monoclonal antibodies directed against NK cell markers (CD57, CD16, and CD56) were not different between women with and without endometriosis. In women with endometriosis, 12.7, 9.5, and 28.8% of lymphocytes were CD57, CD16, and CD56 positive, respectively.
PFMC consisted mainly of phagocytic and human leukocyte antigen (HLA)-restricted or HLA unrestricted cytotoxic cells capable of reacting to various antigens entering the cavity from the lower genital tractus. Furthermore, the decreased NK activity reported in PB and PF of women with endometriosis was not likely to be caused by a quantitative defect, since the percentage of NK positive lymphocytes was not different between women with and without endometriosis.
我们研究了患有和未患有子宫内膜异位症的女性外周血（PB）和腹腔液（PF）中的淋巴细胞亚群，以评估子宫内膜异位症女性自然杀伤（NK）介导的细胞毒性降低是否归因于数量缺陷。
通过流式细胞术分析了59名因疼痛和/或不孕接受诊断性腹腔镜检查的女性的PB和PF单核细胞。
与未患子宫内膜异位症的女性相比，患子宫内膜异位症的女性PF单核细胞（MC）的数量和浓度增加。单核细胞/巨噬细胞标志物（CD14）分别在70.3%和66.9%的患和未患子宫内膜异位症女性的PFMC上表达。PF中的CD4/CD8比值倒置，在患子宫内膜异位症的女性中更为明显。在患子宫内膜异位症女性的PF中，41.3%的淋巴细胞CD8呈阳性，而未患子宫内膜异位症的女性为34.3%。使用三种针对NK细胞标志物（CD57、CD16和CD56）的不同单克隆抗体，患和未患子宫内膜异位症的女性PF中NK阳性淋巴细胞的百分比没有差异。在患子宫内膜异位症的女性中，分别有12.7%、9.5%和28.8%的淋巴细胞CD57、CD16和CD56呈阳性。
PFMC主要由吞噬细胞和能够对从下生殖道进入腔内的各种抗原作出反应的人类白细胞抗原（HLA）限制性或HLA非限制性细胞毒性细胞组成。此外，子宫内膜异位症女性PB和PF中报道的NK活性降低不太可能是由数量缺陷引起的，因为患和未患子宫内膜异位症的女性之间NK阳性淋巴细胞的百分比没有差异。
