通过甘露聚糖酶催化水解提高油棕仁饼中的可发酵甘露糖含量以生产生物丁醇。
Enhanced mannan-derived fermentable sugars of palm kernel cake by mannanase-catalyzed hydrolysis for production of biobutanol.
机构信息
Department of Chemical and Process Engineering, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia; School of Bioprocess Engineering, Universiti Malaysia Perlis, Kompleks Pusat Pengajian Jejawi 3, 02600 Arau, Perlis, Malaysia.
Department of Microbiology, Masjed Soleyman Branch, Islamic Azad University, Masjed Soleyman, Iran.
出版信息
Bioresour Technol. 2016 Oct;218:257-64. doi: 10.1016/j.biortech.2016.06.084. Epub 2016 Jun 24.
Catalytic depolymerization of mannan composition of palm kernel cake (PKC) by mannanase was optimized to enhance the release of mannan-derived monomeric sugars for further application in acetone-butanol-ethanol (ABE) fermentation. Efficiency of enzymatic hydrolysis of PKC was studied by evaluating effects of PKC concentration, mannanase loading, hydrolysis pH value, reaction temperature and hydrolysis time on production of fermentable sugars using one-way analysis of variance (ANOVA). The ANOVA results revealed that all factors studied had highly significant effects on total sugar liberated (P<0.01). The optimum conditions for PKC hydrolysis were 20% (w/v) PKC concentration, 5% (w/w) mannanase loading, hydrolysis pH 4.5, 45°C temperature and 72h hydrolysis time. Enzymatic experiments in optimum conditions revealed total fermentable sugars of 71.54±2.54g/L were produced including 67.47±2.51g/L mannose and 2.94±0.03g/L glucose. ABE fermentation of sugar hydrolysate by Clostridium saccharoperbutylacetonicum N1-4 resulted in 3.27±1.003g/L biobutanol.
通过甘露聚糖酶对棕榈仁饼（PKC）中的甘露聚糖成分进行催化解聚，以提高甘露聚糖衍生的单糖的释放量，从而进一步应用于丙酮丁醇乙醇（ABE）发酵。通过评估 PKC 浓度、甘露聚糖酶用量、水解 pH 值、反应温度和水解时间对使用单因素方差分析（ANOVA）生产可发酵糖的影响，研究了 PKC 酶解的效率。ANOVA 结果表明，所有研究的因素对释放的总糖都有非常显著的影响（P<0.01）。PKC 水解的最佳条件为 20%（w/v）PKC 浓度、5%（w/w）甘露聚糖酶用量、水解 pH 值 4.5、45°C 温度和 72h 水解时间。在最佳条件下进行的酶实验，可产生 71.54±2.54g/L 的总可发酵糖，其中包括 67.47±2.51g/L 的甘露糖和 2.94±0.03g/L 的葡萄糖。由丙酮丁醇梭菌 N1-4 发酵糖水解液可得到 3.27±1.003g/L 的生物丁醇。
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