Valles Steven M, Oi David H, Becnel James J, Wetterer James K, LaPolla John S, Firth Andrew E
Center for Medical, Agricultural and Veterinary Entomology, USDA-ARS, 1600 SW 23rd Drive, Gainesville, FL 32608, USA.
Center for Medical, Agricultural and Veterinary Entomology, USDA-ARS, 1600 SW 23rd Drive, Gainesville, FL 32608, USA.
Virology. 2016 Sep;496:244-254. doi: 10.1016/j.virol.2016.06.014. Epub 2016 Jun 30.
We report the discovery of Nylanderia fulva virus 1 (NfV-1), the first virus identified and characterized from the ant, Nylanderia fulva. The NfV-1 genome (GenBank accession KX024775) is 10,881 nucleotides in length, encoding one large open reading frame (ORF). Helicase, protease, RNA-dependent RNA polymerase, and jelly-roll capsid protein domains were recognized within the polyprotein. Phylogenetic analysis placed NfV-1 in an unclassified clade of viruses. Electron microscopic examination of negatively stained samples revealed particles with icosahedral symmetry with a diameter of 28.7±1.1nm. The virus was detected by RT-PCR in larval, pupal, worker and queen developmental stages. However, the replicative strand of NfV-1 was only detected in larvae. Vertical transmission did not appear to occur, but horizontal transmission was facile. The inter-colonial field prevalence of NfV-1 was 52±35% with some local infections reaching 100%. NfV-1 was not detected in limited samples of other Nylanderia species or closely related ant species.
我们报告了富尔维尼氏蚁病毒1（NfV-1）的发现，这是从富尔维尼氏蚁中鉴定和表征的第一种病毒。NfV-1基因组（GenBank登录号KX024775）长度为10,881个核苷酸，编码一个大的开放阅读框（ORF）。在多聚蛋白中识别出解旋酶、蛋白酶、RNA依赖性RNA聚合酶和果冻卷衣壳蛋白结构域。系统发育分析将NfV-1置于一个未分类的病毒进化枝中。对负染样品的电子显微镜检查揭示了直径为28.7±1.1nm的具有二十面体对称性的颗粒。通过RT-PCR在幼虫、蛹、工蚁和蚁后的发育阶段检测到该病毒。然而，仅在幼虫中检测到NfV-1的复制链。垂直传播似乎未发生，但水平传播很容易。NfV-1在蚁群间的野外流行率为52±35%，一些局部感染率达到100%。在其他富尔维尼氏蚁物种或近缘蚁种的有限样本中未检测到NfV-1。
