Samita Fidelis, Ochieng Charles Otieno, Owuor Philip Okinda, Manguro Lawrence Onyango Arot, Midiwo Jacob Ogweno
a Department of Chemistry, School of Physical and Biological Sciences , Maseno University , Maseno , Kenya.
b Department of Chemistry, School of Biological and Physical Sciences , University of Nairobi , Nairobi , Kenya.
Nat Prod Res. 2017 Mar;31(5):529-536. doi: 10.1080/14786419.2016.1201666. Epub 2016 Jul 3.
A new β-carboline alkaloid named sacleuximine A (1) together with known compounds palmatine (2), isotetrandrine (3), trans-N-feruloyltyramine (4), trans-N-caffeoyltyramine (5), yangambin (6), syringaresinol (7), sesamin (8), (+) epi-quercitol (9), 4-hydroxybenzaldehyde (10), β-sitosterol (11), quercetin 3-O-rutinoside (12) and myricetin 3-O-β-glucose (1→6) α-rhamnoside (13) have been isolated from methanol extract of Triclisia sacleuxii aerial parts. Compounds 1-10 were evaluated for their cytotoxicity against human adenocarcinoma (HeLa), human hepatocarcinoma (Hep3B) and human breast carcinoma (MCF-7) cells lines and also for antibacterial activities against both Gram-positive and Gram-negative bacteria. The cytotoxicity (IC) values ranged between 0.15 and 36.7 μM while the minimum inhibitory concentrations were found to be in the range of 3.9 and 125 μM, respectively. This is the first report of antibacterial compounds and the isolation of lignans together with a β-carboline alkaloid from T. sacleuxii.
从非洲藤山柳地上部分的甲醇提取物中分离得到了一种新的β-咔啉生物碱，命名为萨克勒昔明A（1），以及已知化合物巴马汀（2）、异粉防己碱（3）、反式-N-阿魏酰酪胺（4）、反式-N-咖啡酰酪胺（5）、洋甘菊素（6）、丁香树脂醇（7）、芝麻素（8）、（+）表儿茶醇（9）、4-羟基苯甲醛（10）、β-谷甾醇（11）、槲皮素3-O-芸香糖苷（12）和杨梅素3-O-β-葡萄糖（1→6）α-鼠李糖苷（13）。对化合物1-10进行了针对人腺癌（HeLa）、人肝癌（Hep3B）和人乳腺癌（MCF-7）细胞系的细胞毒性评估，以及对革兰氏阳性和革兰氏阴性细菌的抗菌活性评估。细胞毒性（IC）值在0.15至36.7μM之间，而最低抑菌浓度分别在3.9至125μM范围内。这是首次报道从非洲藤山柳中分离出具有抗菌活性的化合物以及木脂素和一种β-咔啉生物碱。
