Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan 430074, China.
Department of Biomedical Engineering, Key Laboratory for Biomedical Photonics of Ministry of Education, Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan 430074, China.
Nat Commun. 2016 Jul 4;7:12142. doi: 10.1038/ncomms12142.
The precise annotation and accurate identification of neural structures are prerequisites for studying mammalian brain function. The orientation of neurons and neural circuits is usually determined by mapping brain images to coarse axial-sampling planar reference atlases. However, individual differences at the cellular level likely lead to position errors and an inability to orient neural projections at single-cell resolution. Here, we present a high-throughput precision imaging method that can acquire a co-localized brain-wide data set of both fluorescent-labelled neurons and counterstained cell bodies at a voxel size of 0.32 × 0.32 × 2.0 μm in 3 days for a single mouse brain. We acquire mouse whole-brain imaging data sets of multiple types of neurons and projections with anatomical annotation at single-neuron resolution. The results show that the simultaneous acquisition of labelled neural structures and cytoarchitecture reference in the same brain greatly facilitates precise tracing of long-range projections and accurate locating of nuclei.
精确注释和准确识别神经结构是研究哺乳动物大脑功能的前提条件。神经元和神经回路的方向通常通过将大脑图像映射到粗略的轴向采样平面参考图谱来确定。然而，细胞水平的个体差异可能导致位置误差，并导致无法以单细胞分辨率对神经投射进行定向。在这里，我们提出了一种高通量的精确成像方法，该方法可以在 3 天内以 0.32×0.32×2.0 μm 的体素大小获取单个小鼠大脑中荧光标记神经元和细胞体共定位的全脑数据集。我们获得了具有单细胞分辨率的解剖注释的多种类型神经元和投射的小鼠全脑成像数据集。结果表明，在同一大脑中同时获取标记的神经结构和细胞结构参考大大促进了长程投射的精确追踪和核的准确定位。
