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从澳大利亚奇亚籽中提取的分离蛋白的物理化学性质和功能特性。

Physicochemical and functional properties of protein isolate produced from Australian chia seeds.

作者信息

Timilsena Yakindra Prasad, Adhikari Raju, Barrow Colin J, Adhikari Benu

机构信息

School of Applied Sciences, RMIT University, VIC 3083, Australia; CSIRO Materials Science and Engineering, Clayton South, VIC 3169, Australia.

Centre for Chemistry and Biotechnology, Deakin University, Geelong, VIC 3217, Australia.

出版信息

Food Chem. 2016 Dec 1;212:648-56. doi: 10.1016/j.foodchem.2016.06.017. Epub 2016 Jun 11.

Abstract

Protein was isolated from Australian chia seeds and converted to powders using spray, freeze and vacuum drying methods, to investigate the effect of drying methods on physicochemical and functional attributes of chia-seed protein isolate (CPI). It was found that there was no significant difference in the proximate composition; however vacuum dried CPI (VDCPI) had the highest bulk density and oil absorption capacity, whereas spray dried powder (SDCPI) demonstrated the highest solubility, water absorption capacity and lowest surface hydrophobicity. Solubility of all powders was higher at elevated temperature and alkaline pH. Foaming capacity and foam stability of CPI were found to increase with increasing pH and protein concentration. SDCPI was the least denatured and VDCPI the most denatured, demonstrating the poorest solubility and foaming properties of the latter. These findings are expected to be useful in selection of a drying process to yield chia seed protein powders with more desirable functionality.

摘要

从澳大利亚奇亚籽中分离出蛋白质,并采用喷雾干燥、冷冻干燥和真空干燥方法将其制成粉末,以研究干燥方法对奇亚籽分离蛋白(CPI)理化性质和功能特性的影响。结果发现,在近似组成方面没有显著差异;然而,真空干燥的CPI(VDCPI)具有最高的堆积密度和吸油能力,而喷雾干燥粉末(SDCPI)表现出最高的溶解度、吸水能力和最低的表面疏水性。所有粉末在高温和碱性pH条件下的溶解度更高。发现CPI的发泡能力和泡沫稳定性随pH值和蛋白质浓度的增加而增加。SDCPI变性程度最小,VDCPI变性程度最大,表明后者的溶解度和发泡性能最差。这些研究结果有望有助于选择干燥工艺,以生产出具有更理想功能的奇亚籽蛋白粉。

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