1. Department of Bioengineering, Rice University, Houston, TX 77005, USA.; 2. State Key Laboratory of Medicinal Chemical Biology, College of Life Sciences, Nankai University, Tianjin 300071, China;
1. Department of Bioengineering, Rice University, Houston, TX 77005, USA.
Theranostics. 2016 Jun 15;6(9):1353-61. doi: 10.7150/thno.16093. eCollection 2016.
Accurate and reliable quantification of biomarkers in the blood is essential in disease screening and diagnosis. Here we describe an iron oxide nanoparticle (IONP)-linked immunosorbent assay (ILISA) for detecting biomolecules in human serum. Sandwich ILISA was optimized for the detection of four important serological markers, IgA, IgG, IgM, and C-reactive protein (CRP), and assessed with normal sera, simulated disease-state sera and the serum samples from patients infected with West Nile virus (WNV) or human herpes virus (HHV). Our study shows that using the detection assay formulated with 18.8 nm wüstite nanocrystals, ILISA can achieve sub-picomolar detection sensitivity, and all four markers can be accurately quantified over a large dynamic range. In addition, ILISA is not susceptible to variations in operating procedures and shows better linearity and higher stability compared with ELISA, which facilitates its integration into detection methods suitable for point of care. Our results demonstrate that ILISA is a simple and versatile nanoplatform for highly sensitive and reliable detection of serological biomarkers in biomedical research and clinical applications.
准确可靠地定量检测血液中的生物标志物对于疾病的筛查和诊断至关重要。在这里，我们描述了一种用于检测人血清中生物分子的氧化铁纳米颗粒（IONP）-酶联免疫吸附测定法（ILISA）。我们对夹心 ILISA 进行了优化，以检测四种重要的血清学标志物，即 IgA、IgG、IgM 和 C 反应蛋白（CRP），并使用正常血清、模拟疾病状态血清以及感染西尼罗河病毒（WNV）或人类疱疹病毒（HHV）的患者的血清样本进行了评估。我们的研究表明，使用 18.8nm 磁铁矿纳米晶体制成的检测方法，ILISA 可以实现亚皮摩尔级别的检测灵敏度，并且可以在很大的动态范围内准确地定量检测这四种标志物。此外，与 ELISA 相比，ILISA 不受操作程序变化的影响，显示出更好的线性和更高的稳定性，这使其更易于集成到适合即时护理的检测方法中。我们的结果表明，ILISA 是一种简单而多功能的纳米平台，可用于生物医学研究和临床应用中血清生物标志物的高灵敏度和可靠检测。
