以葡聚糖作为佐剂的杜氏利什曼原虫抗原的保护作用。

Protective effect of L. donovani antigens using glucan as an adjuvant.

作者信息

Obaid K A, Ahmad S, Khan H M, Mahdi A A, Khanna R

机构信息

Department of Microbiology, Jawaharlal Nehru Medical College, Aligarh Muslim University, India.

出版信息

Int J Immunopharmacol. 1989;11(3):229-35. doi: 10.1016/0192-0561(89)90159-8.

DOI:10.1016/0192-0561(89)90159-8

PMID:2737802

Abstract

Golden hamsters were immunized with various antigen fractions of Leishmania donovani promastigotes. Beta 1,3-glucan was used as an adjuvant in these vaccination experiments. The results indicate that immunization of animals with the microsomal fraction (subcellular fraction III) in combination with glucan confers considerable immune protection against L. donovani infection. The immune protection was confirmed by correspondingly lower parasite burden in the livers and spleens of test animals compared to controls. Additionally, the vaccinated animals showed positive skin test responsiveness after challenge, along with increased antibody titres. Immunization of animals with whole and particulate antigen fractions was also found to afford a high degree of resistance. The other subcellular and soluble antigen fractions conferred very little protection. In these experiments, glucan was found to be a potent adjuvant when injected, intraperitoneally, with Leishmania antigens. Similar doses of parasite extracts given without an adjuvant were able to confer only very little or no protection.

摘要

用杜氏利什曼原虫前鞭毛体的各种抗原组分对金黄仓鼠进行免疫。在这些疫苗接种实验中，β-1,3-葡聚糖用作佐剂。结果表明，用微粒体组分（亚细胞组分III）与葡聚糖联合免疫动物，可对杜氏利什曼原虫感染提供相当程度的免疫保护。与对照组相比，试验动物肝脏和脾脏中相应较低的寄生虫负荷证实了这种免疫保护作用。此外，接种疫苗的动物在受到攻击后皮肤试验反应呈阳性，抗体滴度也有所增加。还发现用完整和颗粒性抗原组分免疫动物可提供高度的抵抗力。其他亚细胞和可溶性抗原组分提供的保护作用很小。在这些实验中，发现葡聚糖与利什曼原虫抗原腹腔注射时是一种有效的佐剂。不使用佐剂给予相似剂量的寄生虫提取物只能提供很少或没有保护作用。