Lee Su-Hwa, Kim Sang-Soo, Lee Dong-Hun, Kim Ah-Ra, Quan Fu-Shi
Department of Biomedical Science, Graduate School, Kyung Hee University, Seoul, South Korea.
Department of Medical Zoology, Kyung Hee University School of Medicine, Seoul, South Korea.
Parasit Vectors. 2016 Jul 4;9(1):384. doi: 10.1186/s13071-016-1662-7.
The frequent outbreaks of human trichinellosis globally underscore the need to develop effective vaccine. We hypothesized that a novel vaccine could improve vaccine efficacy against Trichinella spiralis.
In this study, we developed virus-like particles (VLPs) containing the 53 KDa excretory/secretory (ES) protein of T. spiralis and the influenza matrix protein 1 (M1) as a core protein, and investigated the protective efficacy of the VLPs alone or with cholera toxin (CT) in a mouse model.
Intramuscular immunization induced T. spiralis-specific IgG, IgG1 and IgG2a antibody responses before and after challenge infections in the sera. These antibody responses were significantly enhanced in mice immunized with adjuvanted VLPs. Upon challenge infection, vaccinated mice showed significantly reduced worm burden in the diaphragm. Protective immune responses and efficacy of protection were significantly improved by immunization with VLPs together with CT adjuvant.
Our results are informative for a better understanding of the protective immunity induced by T. spiralis VLPs, and will provide insight into designing safe and effective vaccines.
全球范围内人旋毛虫病的频繁爆发凸显了开发有效疫苗的必要性。我们推测一种新型疫苗可能会提高针对旋毛虫的疫苗效力。
在本研究中，我们构建了含有旋毛虫53 kDa排泄/分泌（ES）蛋白和流感病毒基质蛋白1（M1）作为核心蛋白的病毒样颗粒（VLP），并在小鼠模型中研究了单独的VLP或与霍乱毒素（CT）联合使用时的保护效力。
肌肉注射免疫在攻击感染前后均诱导了血清中针对旋毛虫的特异性IgG、IgG1和IgG2a抗体反应。在用佐剂VLP免疫的小鼠中，这些抗体反应显著增强。在攻击感染后，接种疫苗的小鼠膈肌中的虫负荷显著降低。与CT佐剂一起接种VLP可显著改善保护性免疫反应和保护效力。
我们的结果有助于更好地理解旋毛虫VLP诱导的保护性免疫，将为设计安全有效的疫苗提供见解。
