Stover F S
Central Research Laboratories, Monsanto Co., St. Louis, MO 63167.
J Chromatogr. 1989 May 24;470(1):201-8. doi: 10.1016/s0021-9673(00)94213-4.
Performance of narrow range ampholytes and a discrete spacer mixture is evaluated for improved protein separations by cationic isotachophoresis. A spacer mixture containing 22 cations is developed and relative step heights of components are presented. Different ampholytes and the discrete spacer give unique results for test mixtures of model proteins. While no spacer mixture can be universally recommended, discrete spacers offer the possibility of optimizing separations based on component selection. An example of optimizing a separation of five model proteins is presented.
通过阳离子等速电泳评估窄范围两性电解质和离散间隔物混合物对改善蛋白质分离的性能。开发了一种包含22种阳离子的间隔物混合物，并给出了各组分的相对台阶高度。不同的两性电解质和离散间隔物对模型蛋白质测试混合物产生独特的结果。虽然没有一种间隔物混合物能被普遍推荐，但离散间隔物提供了基于组分选择优化分离的可能性。给出了一个优化五种模型蛋白质分离的示例。
