Mattingly D, Martin H, Tyler C
Postgraduate Medical School, University of Exeter.
J Clin Pathol. 1989 Jun;42(6):661-6. doi: 10.1136/jcp.42.6.661.
The simultaneous estimation of steroids in plasma was carried out by the assay of cortisol, corticosterone, and testosterone. The method entails separation by means of thin layer chromatography, followed by conversion to a fluorophore and fluorimetric measurement. Its major advantages are its high specificity, its ability to detect unknown substances, and the ease with which it can be performed. The method has acceptable levels of accuracy and precision and the normal values obtained by it compare well with those given by methods in general use.
通过对皮质醇、皮质酮和睾酮进行测定，同时对血浆中的类固醇进行了评估。该方法需要通过薄层色谱法进行分离，随后转化为荧光团并进行荧光测定。其主要优点是特异性高、能够检测未知物质以及操作简便。该方法具有可接受的准确度和精密度水平，通过它获得的正常值与一般使用的方法所给出的正常值相比良好。
