Mattingly D, Martin H, Tyler C M
Postgraduate Medical School, University of Exeter.
J Clin Pathol. 1993 Dec;46(12):1109-12. doi: 10.1136/jcp.46.12.1109.
To develop a fluorimetric method for the estimation of urinary aldosterone; to establish a normal range in 24 hour and overnight urine samples; and to investigate the use of overnight urines for detecting hyperaldosteronism.
Essential steps include hydrolysis of the 18 conjugate to release aldosterone and its oxidation with Benedict's solution, followed by thin-layer chromatography on silica gel and development of fluorescence on the plate with sulphuric acid.
There was a linear correlation between the amount of aldosterone and the area under the peak on the chromatogram. The mean intra-assay and interassay coefficients of variation were 4.4% and 6.8%, respectively. The mean aldosterone excretion in 67 adults was 15.7 (SD 8.1) nmol/24 hours. The mean overnight excretion in 65 adults was 2.6 (1.4) nmol/8 hours. The method detected raised concentrations in patients with primary and secondary aldosteronism.
This technique provides an accurate means of assaying urinary aldosterone. Overnight estimations seem to be as effective as 24 hour assays for identifying patients with hyperaldosteronism.
开发一种用于估算尿醛固酮的荧光法；确定24小时和过夜尿样的正常范围；并研究使用过夜尿样检测醛固酮增多症。
基本步骤包括将18-共轭物水解以释放醛固酮，并用本尼迪特溶液将其氧化，随后在硅胶上进行薄层色谱分析，并用硫酸在板上显荧光。
醛固酮量与色谱图上峰下面积之间存在线性相关性。批内和批间变异系数的平均值分别为4.4%和6.8%。67名成年人醛固酮的平均排泄量为15.7（标准差8.1）nmol/24小时。65名成年人过夜排泄量的平均值为2.6（1.4）nmol/8小时。该方法检测到原发性和继发性醛固酮增多症患者的浓度升高。
该技术提供了一种准确测定尿醛固酮的方法。对于识别醛固酮增多症患者，过夜测定似乎与24小时测定同样有效。
