Wells Adam J, Hoffman Jay R, Jajtner Adam R, Varanoske Alyssa N, Church David D, Gonzalez Adam M, Townsend Jeremy R, Boone Carleigh H, Baker Kayla M, Beyer Kyle S, Mangine Gerald T, Oliveira Leonardo P, Fukuda David H, Stout Jeffrey R
Institute of Exercise Physiology and Wellness, Educational and Human Sciences, University of Central Florida, 12494 University Blvd, Orlando, FL 32816, USA.
Department of Health Professions, Hofstra University, Hempstead, NY 11549, USA.
Nutrients. 2016 Jul 2;8(7):409. doi: 10.3390/nu8070409.
The recruitment and infiltration of classical monocytes into damaged muscle is critical for optimal tissue remodeling. This study examined the effects of an amino acid supplement on classical monocyte recruitment following an acute bout of lower body resistance exercise. Ten resistance-trained men (24.7 ± 3.4 years; 90.1 ± 11.3 kg; 176.0 ± 4.9 cm) ingested supplement (SUPP) or placebo (PL) immediately post-exercise in a randomized, cross-over design. Blood samples were obtained at baseline (BL), immediately (IP), 30-min (30P), 1-h (1H), 2-h (2H), and 5-h (5H) post-exercise to assess plasma concentrations of monocyte chemoattractant protein 1 (MCP-1), myoglobin, cortisol and insulin concentrations; and expressions of C-C chemokine receptor-2 (CCR2), and macrophage-1 antigen (CD11b) on classical monocytes. Magnitude-based inferences were used to provide inferences on the true effects of SUPP compared to PL. Changes in myoglobin, cortisol, and insulin concentrations were similar between treatments. Compared to PL, plasma MCP-1 was "very likely greater" (98.1% likelihood effect) in SUPP at 2H. CCR2 expression was "likely greater" at IP (84.9% likelihood effect), "likely greater" at 1H (87.7% likelihood effect), "very likely greater" at 2H (97.0% likelihood effect), and "likely greater" at 5H (90.1% likelihood effect) in SUPP, compared to PL. Ingestion of SUPP did not influence CD11b expression. Ingestion of an amino acid supplement immediately post-exercise appears to help maintain plasma MCP-1 concentrations and augment CCR2 expression in resistance trained men.
经典单核细胞募集并浸润到受损肌肉中对于实现最佳组织重塑至关重要。本研究检测了一次急性下肢抗阻运动后补充氨基酸对经典单核细胞募集的影响。十名经过抗阻训练的男性（年龄24.7±3.4岁；体重90.1±11.3千克；身高176.0±4.9厘米）在运动后立即以随机交叉设计摄入补充剂（SUPP）或安慰剂（PL）。在运动前基线（BL）、运动后即刻（IP）、30分钟（30P）、1小时（1H）、2小时（2H）和5小时（5H）采集血样，以评估血浆中单核细胞趋化蛋白1（MCP-1）、肌红蛋白、皮质醇和胰岛素的浓度；以及经典单核细胞上C-C趋化因子受体2（CCR2）和巨噬细胞-1抗原（CD11b）的表达。基于量级的推断用于推断SUPP与PL相比的真实效果。两种处理之间肌红蛋白、皮质醇和胰岛素浓度的变化相似。与PL相比，SUPP组在2小时时血浆MCP-1“极有可能更高”（效应可能性为98.1%）。与PL相比，SUPP组在IP时CCR2表达“有可能更高”（效应可能性为84.9%），在1小时时“有可能更高”（效应可能性为87.7%），在2小时时“极有可能更高”（效应可能性为97.0%），在5小时时“有可能更高”（效应可能性为90.1%）。摄入SUPP不影响CD11b表达。运动后立即摄入氨基酸补充剂似乎有助于维持抗阻训练男性的血浆MCP-1浓度并增加CCR2表达。
