Department of Exercise Physiology, Kent State University, Kent, OH.
Exercise and Nutrition Science, Lipscomb University, Nashville, TN.
Med Sci Sports Exerc. 2018 Nov;50(11):2231-2241. doi: 10.1249/MSS.0000000000001703.
To examine the impact of polyphenol supplementation on the recruitment, mobilization, and activation of monocyte subsets after resistance exercise.
Thirty-eight recreationally active males (22.1 ± 3.1 yr; 173.9 ± 7.9 cm; 77.8 ± 14.5 kg) were assigned to 28 d of polyphenol blend (PPB) supplementation, placebo (PL), or control (CON). Blood samples were obtained before (PRE) postresistance exercise, immediately (IP) postresistance exercise, 1 h (1H) postresistance exercise, 5 h (5H) postresistance exercise, 24 h (24H) postresistance exercise, and 48 h (48H) postresistance exercise (PPB/PL) or rest (CON). Fine-needle biopsies were obtained from the vastus lateralis at PRE, 1H, 5H, and 48H. Circulating concentrations of macrophage chemoattractant protein-1 (MCP-1) and fractalkine, as well as intramuscular MCP-1 were analyzed via multiplex assay. Changes in the proportions and expression of CD11b on monocyte subsets were assessed via flow cytometry.
Circulating MCP-1 increased in PPB and PL at IP with further increases at 5H. Intramuscular MCP-1 was increased at 1H, 5H, and 48H in all groups. Classical monocyte proportions were reduced in PPB and PL at IP, and increased at 1H. Nonclassical monocytes were increased in PPB and PL at IP, whereas intermediate monocytes were increased at IP, and reduced at 1H. Intermediate monocytes were increased in PPB at 24H and 48H. CD11b expression was reduced on PPB compared with PL and CON at PRE on intermediate and nonclassical monocytes.
Resistance exercise may elicit selective mobilization of intermediate monocytes at 24H and 48H, which may be mediated by tissue damage. Additionally, polyphenol supplementation may suppress CD11b expression on monocyte subsets at rest.
研究多酚补充剂对抗阻运动后单核细胞亚群募集、动员和激活的影响。
38 名有规律运动的男性（22.1±3.1 岁；173.9±7.9cm；77.8±14.5kg）分为多酚混合物（PPB）补充组、安慰剂（PL）组和对照组（CON），每组 19 人。在抗阻运动前（PRE）、运动即刻（IP）、运动后 1 小时（1H）、运动后 5 小时（5H）、运动后 24 小时（24H）和运动后 48 小时（48H）采集血液样本（PPB/PL）或休息（CON）。在 PRE、1H、5H 和 48H 时从股外侧肌采集细针活检。通过多重分析测定循环巨噬细胞趋化蛋白-1（MCP-1）和 fractalkine 浓度以及肌内 MCP-1。通过流式细胞术评估单核细胞亚群 CD11b 的比例和表达变化。
PPB 和 PL 在 IP 时循环 MCP-1 增加，5H 时进一步增加。所有组的肌内 MCP-1 在 1H、5H 和 48H 时增加。PPB 和 PL 在 IP 时经典单核细胞比例降低，1H 时增加。非经典单核细胞在 IP 时增加，而中间单核细胞在 IP 时增加，在 1H 时减少。中间单核细胞在 24H 和 48H 时在 PPB 中增加。与 PL 和 CON 相比，PPB 在 PRE 时中间和非经典单核细胞上的 CD11b 表达降低。
抗阻运动可能会在 24H 和 48H 时选择性动员中间单核细胞，这可能是由组织损伤引起的。此外，多酚补充可能会在休息时抑制单核细胞亚群的 CD11b 表达。
