高强度大运动量抗阻运动后的单核细胞募集

Monocyte Recruitment after High-Intensity and High-Volume Resistance Exercise.

作者信息

Wells Adam J, Hoffman Jay R, Jajtner Adam R, Varanoske Alyssa N, Church David D, Gonzalez Adam M, Townsend Jeremy R, Boone Carleigh H, Baker Kayla M, Beyer Kyle S, Mangine Gerald T, Oliveira Leonardo P, Fukuda David H, Stout Jeffrey R

机构信息

1School of Health and Kinesiology, Georgia Southern University, Statesboro, GA; 2Institute of Exercise Physiology and Wellness; University of Central Florida, Orlando, FL; 3Department of Health Professions, Hofstra University, Hempstead, NY; and 4Department of Exercise Science and Sport Management, Kennesaw State University, Kennesaw, GA.

出版信息

Med Sci Sports Exerc. 2016 Jun;48(6):1169-78. doi: 10.1249/MSS.0000000000000878.

DOI:10.1249/MSS.0000000000000878

PMID:26784277

Abstract

UNLABELLED

The innate immune response is generally considered to have an important role in tissue remodeling after resistance exercise.

PURPOSE

The purpose of this study was to compare changes in markers of monocyte recruitment after an acute bout of high-intensity (HVY) versus high-volume (VOL) lower-body resistance exercise.

METHODS

Ten resistance-trained men (24.7 ± 3.4 yr, 90.1 ± 11.3 kg, 176.0 ± 4.9 cm) performed each protocol in a randomized, counterbalanced order. Blood samples were collected at baseline, immediately (IP), 30 min (30P), 1 h (1H), 2 h (2H), and 5 h (5H) postexercise. Plasma concentrations of monocyte chemoattractant protein 1 (MCP-1), tumor necrosis factor alpha (TNF-α), myoglobin, and cortisol were measured via assay. Tumor necrosis factor receptor 1 (TNFr1), macrophage-1 antigen (cluster of differentiation 11b [CD11b]), and C-C chemokine receptor 2 (CCR2) expression levels were measured using flow cytometry. TNFr1 and CD11b were assessed on CD14CD16 monocytes, whereas CCR2 was assessed on CD14 monocytes.

RESULTS

Plasma myoglobin concentrations were significantly greater after HVY compared with VOL (P < 0.001). Changes in plasma TNF-α, MCP-1, and expression levels of CCR2 and CD11b were similar between HVY and VOL. When collapsed across groups, TNF-α was significantly increased at IP, 30P, 1H, and 2H (P values < 0.05), whereas MCP-1 was significantly elevated at all postexercise time points (P values < 0.05). CCR2 expression on CD14 monocytes was significantly lower at IP, 1H, 2H, and 5H (P values < 0.05). CD11b expression on CD14 CD16 was significantly greater at IP (P < 0.014) and 1H (P = 0.009). TNFr1 expression did not differ from baseline at any time point. Plasma cortisol concentrations did not seem to be related to receptor expression.

CONCLUSIONS

Results indicate that both HVY and VOL protocols stimulate a robust proinflammatory response. However, no differences were noted between resistance exercise training paradigms.

摘要

未标注

一般认为先天免疫反应在抗阻运动后的组织重塑中起重要作用。

目的

本研究的目的是比较一次急性高强度（HVY）与高容量（VOL）下肢抗阻运动后单核细胞募集标志物的变化。

方法

10名抗阻训练男性（24.7±3.4岁，90.1±11.3千克，176.0±4.9厘米）以随机、交叉的顺序进行每种方案。在运动前基线、运动后即刻（IP）、30分钟（30P）、1小时（1H）、2小时（2H）和5小时（5H）采集血样。通过检测测定血浆中单核细胞趋化蛋白1（MCP-1）、肿瘤坏死因子α（TNF-α）、肌红蛋白和皮质醇的浓度。使用流式细胞术测量肿瘤坏死因子受体1（TNFr1）、巨噬细胞-1抗原（分化簇11b [CD11b]）和C-C趋化因子受体2（CCR2）的表达水平。在CD14CD16单核细胞上评估TNFr1和CD11b，而在CD14单核细胞上评估CCR2。

结果

与VOL相比，HVY后血浆肌红蛋白浓度显著更高（P<0.001）。HVY和VOL之间血浆TNF-α、MCP-1以及CCR2和CD11b的表达水平变化相似。当合并各分组时，TNF-α在IP、30P、1H和2H时显著升高（P值<0.05），而MCP-1在所有运动后时间点均显著升高（P值<0.05）。CD14单核细胞上CCR2的表达在IP、1H、2H和5H时显著降低（P值<0.05）。CD14 CD16上CD11b的表达在IP时显著更高（P<0.014），在1H时显著更高（P = 0.009）。TNFr1的表达在任何时间点与基线相比均无差异。血浆皮质醇浓度似乎与受体表达无关。