一种用于测量细胞内 Na(+) 和 K(+) 的高灵敏度 HPLC 分析方法及其在感染疟原虫的红细胞中的应用。
A high-sensitivity HPLC assay for measuring intracellular Na(+) and K(+) and its application to Plasmodium falciparum infected erythrocytes.
机构信息
Research School of Biology, Australian National University, Canberra, ACT 2601, Australia.
Centre for Tropical Medicine and Global Health, Nuffield Department of Medicine, University of Oxford, UK.
出版信息
Sci Rep. 2016 Jul 7;6:29241. doi: 10.1038/srep29241.
The measurement of intracellular ion concentrations, and the screening of chemical agents to identify molecules targeting ion transport, has traditionally involved low-throughput techniques. Here we present a novel HPLC method that allows the rapid, high-sensitivity measurement of cell Na(+) and K(+) content, demonstrating its utility by monitoring the ionic changes induced in the intracellular malaria parasite by the new spiroindolone antimalarial KAE609.
细胞内离子浓度的测量，以及筛选针对离子转运的化学试剂的分子，传统上都涉及低通量技术。在这里，我们提出了一种新颖的 HPLC 方法，可实现细胞内 Na(+)和 K(+)含量的快速、高灵敏度测量，通过监测新的螺环吲哚酮抗疟药 KAE609 引起的疟原虫内离子变化，证明了其有用性。
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