Department of Bioelectronics, Ecole Nationale Supérieure des Mines, CMP-EMSE, MOC, 13541, Gardanne, France.
Department of Electrical Engineering, University of California, Santa Cruz, CA, 95064, USA.
Adv Healthc Mater. 2016 Sep;5(17):2295-302. doi: 10.1002/adhm.201600494. Epub 2016 Jul 7.
A compact multianalyte biosensing platform is reported, composed of an organic electrochemical transistor (OECT) microarray integrated with a pumpless "finger-powered" microfluidic, for quantitative screening of glucose, lactate, and cholesterol levels. A biofunctionalization method is designed, which provides selectivity towards specific metabolites as well as minimization of any background interference. In addition, a simple method is developed to facilitate multi-analyte sensing and avoid electrical crosstalk between the different transistors by electrically isolating the individual devices. The resulting biosensing platform, verified using human samples, offers the possibility to be used in easy-to-obtain biofluids with low abundance metabolites, such as saliva. Based on our proposed method, other types of enzymatic biosensors can be integrated into the array to achieve multiplexed, noninvasive, personalized point-of-care diagnostics.
本文报道了一种紧凑的多分析物生物传感平台，由有机电化学晶体管 (OECT) 微阵列与无泵“手指驱动”微流控集成而成，用于定量筛选葡萄糖、乳酸和胆固醇水平。设计了一种生物功能化方法，该方法对特定代谢物具有选择性，并最大限度地减少任何背景干扰。此外，还开发了一种简单的方法来促进多分析物传感，并通过电隔离各个器件来避免不同晶体管之间的电串扰。使用人体样本验证的生物传感平台，有可能用于容易获得的低丰度代谢物的生物流体中，例如唾液。基于我们提出的方法，可以将其他类型的酶生物传感器集成到阵列中，以实现多路复用、非侵入性、个性化的即时诊断。
