Ali Md Wajed, Alauddin Md, Azad Md Thoufic Anam, Hasan Md Ariful, Appiah-Kwarteng Cornelia, Takasu Masaki, Baba Minami, Kitoh Katsuya, Rahman Moizur, Takashima Yasuhiro
Department of Animal Husbandry and Veterinary Science, Faculty of Agriculture, University of Rajshahi, Rajshahi-6205, Bangladesh.
J Vet Med Sci. 2016 Nov 1;78(10):1577-1582. doi: 10.1292/jvms.16-0080. Epub 2016 Jul 11.
An epidemiological survey of Theileria annulata infection was undertaken in a cattle population in Rajshahi Division, Bangladesh. The local cattle breeds from the area (North Bengal Gray and Deshi) and crosses between the local breeds and Holstein cattle were predominantly screened. In total, 192 cattle serum samples were collected in two areas of Rajshahi Division, the Rajshahi District (n=147) and Natore District (n=45). The samples were screened with an enzyme-linked immunosorbent assay using T. annulata surface protein (TaSP) as the antigen. The seroprevalence was 80.0% (36/45) in Natore and 20.4% (30/147) in Rajshahi. A logistic regression analysis showed that the sampling location was significantly associated with seropositivity, whereas age, sex and breed were not. Although the logistic regression analysis did not show a linear dependence on age, we considered age-specific seroprevalence separately in the two districts. Seroprevalence did not differ significantly among age categories in the Natore District. In contrast, all the cattle <1 year old in the Rajshahi District were seronegative (11/11). Seroprevalence in the 1- and 2-year-old cattle was significantly lower in the Rajshahi District than in the Natore District. In the older age categories (3, 4 and >5 years), seroprevalence did not differ significantly between the Natore and Rajshahi Districts. These results suggest that the cattle in the Rajshahi District were sporadically exposed to T. annulata, whereas most cattle in the Natore District became infected during an early phase of life.
在孟加拉国拉杰沙希专区的一个牛群中开展了环形泰勒虫感染的流行病学调查。主要对该地区的本地牛品种（北孟加拉灰牛和德希牛）以及本地品种与荷斯坦牛的杂交品种进行了筛查。总共在拉杰沙希专区的两个地区，即拉杰沙希县（n = 147）和纳托尔县（n = 45）采集了192份牛血清样本。使用环形泰勒虫表面蛋白（TaSP）作为抗原，通过酶联免疫吸附测定法对样本进行筛查。纳托尔县的血清阳性率为80.0%（36/45），拉杰沙希县为20.4%（30/147）。逻辑回归分析表明，采样地点与血清阳性显著相关，而年龄、性别和品种则不然。尽管逻辑回归分析未显示出对年龄的线性依赖性，但我们分别考虑了两个县按年龄划分的血清阳性率。纳托尔县各年龄组的血清阳性率无显著差异。相比之下，拉杰沙希县所有1岁以下的牛均为血清阴性（11/11）。拉杰沙希县1岁和2岁牛的血清阳性率显著低于纳托尔县。在较大年龄组（3岁、4岁和>5岁）中，纳托尔县和拉杰沙希县的血清阳性率无显著差异。这些结果表明，拉杰沙希县的牛偶尔接触环形泰勒虫，而纳托尔县的大多数牛在生命早期就受到了感染。
