Naundorf A, Caussette M, Ajisaka K
a Meiji Milk Products Co., Ltd.
Biosci Biotechnol Biochem. 1998;62(7):1313-7. doi: 10.1271/bbb.62.1313.
A recombinant β-galactosidase, which was obtained from the β-galactosidase C gene of Bacillus circulans and cleaves the non-reducing end galactosyl residue of β(1→3)-linkages selectively, was immobilized using CNBr-Sepharose. Although the effect of pH was not changed by the immobilization, the thermostability and stability in the presence of DMF were increased. Optimization of the transglycosylation using para-nitrophenyl β-D-galactopyranoside as a donor and benzyl-α-D-N-acetylgalactosaminide as an acceptor afforded a β(1→3)-linked disaccharide derivative with 62% molar yield in a gram scale synthesis. Using the methyl-analogue as an acceptor, 53% of the acceptor was converted to the respective β(1→3)-disaccharide.
一种重组β-半乳糖苷酶，它是从环状芽孢杆菌的β-半乳糖苷酶C基因获得的，能选择性地切割β(1→3)键的非还原端半乳糖基残基，使用溴化氰活化的琼脂糖凝胶（CNBr-Sepharose）将其固定化。尽管固定化后pH的影响没有改变，但热稳定性和在二甲基甲酰胺（DMF）存在下的稳定性有所提高。以对硝基苯基β-D-吡喃半乳糖苷作为供体、苄基-α-D-N-乙酰半乳糖胺作为受体对转糖基化反应进行优化，在克级规模合成中得到了摩尔产率为62%的β(1→3)连接的二糖衍生物。使用甲基类似物作为受体时，53%的受体转化为相应的β(1→3)二糖。
