Ferris C F, Axelson J F, Martin A M, Roberge L F
Department of Physiology, University of Massachusetts Medical Center, Worcester 01655.
Neuroscience. 1989;29(3):675-83. doi: 10.1016/0306-4522(89)90140-1.
When paired for 15-min periods for 5-8 consecutive days, castrated, testosterone-treated hamsters consistently assumed the dominant status, based on a higher aggression index (18 +/- 3) and frequency of flank marking (15 +/- 3) as compared to their castrated, untreated subordinate partners (-1.3 +/- 1 and 2.4 +/- 1, respectively). In addition to these hamsters with established dominant/subordinate relationships, control hamsters with no social interactions were killed, and in all animals the vasopressin level in the anterior hypothalamus-medial preoptic area was assessed by counting vasopressin immunoreactive perikarya following immunocytochemistry, or by radioimmunoassay of vasopressin from tissue punches. In the socialized pairs the subordinate hamsters had a significantly (P less than 0.01) lower number of vasopressin staining perikarya in the anterior hypothalamus, specifically the area of the nucleus circularis, than their dominant partners (n = 6 pairs). There was also a significantly (P less than 0.001) lower level of vasopressin immunoreactivity in punches taken from the area of the nucleus circularis in subordinate hamsters as compared to their dominant partners (n = 14 pairs). However, there were no significant differences in the number of perikarya or the concentration of immunoreactive vasopressin between subordinate and dominant hamsters in the supraoptic nucleus, paraventricular nucleus, suprachiasmatic nucleus or bed nucleus of the stria terminalis. The number of perikarya (n = 5 pairs) and concentration of vasopressin (n = 8 pairs) for all vasopressin immunoreactive sites, including the nucleus circularis, were similar for testosterone-treated and untreated hamsters that remained isolated and not subjected to daily aggressive encounters.(ABSTRACT TRUNCATED AT 250 WORDS)
当阉割后经睾酮处理的仓鼠与未处理的阉割从属仓鼠连续5至8天每天配对15分钟时，前者基于更高的攻击指数（18±3）和胁腹标记频率（15±3），始终占据主导地位，而其从属伙伴的攻击指数和胁腹标记频率分别为-1.3±1和2.4±1。除了这些已建立主导/从属关系的仓鼠外，未进行社交互动的对照仓鼠也被处死，并通过免疫细胞化学计数血管加压素免疫反应性核周体，或通过对组织切片进行血管加压素放射免疫测定，来评估所有动物下丘脑前部-内侧视前区的血管加压素水平。在有社交行为的配对仓鼠中，从属仓鼠下丘脑前部，特别是环核区域的血管加压素染色核周体数量，显著低于其主导伙伴（P<0.01，n = 6对）。与主导伙伴相比，从属仓鼠环核区域切片中的血管加压素免疫反应性水平也显著更低（P<0.001，n = 14对）。然而，在视上核、室旁核、视交叉上核或终纹床核中，从属和主导仓鼠的核周体数量或免疫反应性血管加压素浓度没有显著差异。对于包括环核在内的所有血管加压素免疫反应位点，经睾酮处理和未处理、保持隔离且未经历每日攻击行为的仓鼠，其核周体数量（n = 5对）和血管加压素浓度（n = 8对）相似。（摘要截取自250词）
