Robert Jacques, Jancovich James K
Department of Microbiology and Immunology, University of Rochester Medical Center, Rochester, NY 14642, USA.
Department of Biological Sciences, California State University San Marcos, 333 S. Twin Oaks Valley Rd., San Marcos, CA 92096, USA.
Viruses. 2016 Jul 6;8(7):187. doi: 10.3390/v8070187.
Ranaviruses (Iridoviridae) are large DNA viruses that are causing emerging infectious diseases at an alarming rate in both wild and captive cold blood vertebrate species all over the world. Although the general biology of these viruses that presents some similarities with poxvirus is characterized, many aspects of their replication cycles, host cell interactions and evolution still remain largely unclear, especially in vivo. Over several years, strategies to generate site-specific ranavirus recombinant, either expressing fluorescent reporter genes or deficient for particular viral genes, have been developed. We review here these strategies, the main ranavirus recombinants characterized and their usefulness for in vitro and in vivo studies.
蛙病毒（虹彩病毒科）是大型DNA病毒，在世界各地的野生和圈养冷血脊椎动物物种中，正以惊人的速度引发新出现的传染病。尽管这些病毒的一般生物学特性已得到描述，且与痘病毒有一些相似之处，但其复制周期、宿主细胞相互作用和进化的许多方面在很大程度上仍不清楚，尤其是在体内。多年来，已经开发出了产生位点特异性蛙病毒重组体的策略，这些重组体要么表达荧光报告基因，要么特定病毒基因有缺陷。我们在此综述这些策略、已鉴定的主要蛙病毒重组体及其在体外和体内研究中的用途。
