Esposito Emilia, Lim Bomyi, Guessous Ghita, Falahati Hanieh, Levine Michael
Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey 08544, USA; Department of Molecular and Cell Biology, Division of Genetics, Genomics, and Development, University of California at Berkeley, Berkeley, California 94720, USA;
Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey 08544, USA;
Genes Dev. 2016 Jul 1;30(13):1503-8. doi: 10.1101/gad.281188.116.
Transcriptional repression is a pervasive feature of animal development. Here, we employ live-imaging methods to visualize the Snail repressor, which establishes the boundary between the presumptive mesoderm and neurogenic ectoderm of early Drosophila embryos. Snail target enhancers were attached to an MS2 reporter gene, permitting detection of nascent transcripts in living embryos. The transgenes exhibit initially broad patterns of transcription but are refined by repression in the mesoderm following mitosis. These observations reveal a correlation between mitotic silencing and Snail repression. We propose that mitosis and other inherent discontinuities in transcription boost the activities of sequence-specific repressors, such as Snail.
转录抑制是动物发育过程中普遍存在的一个特征。在此，我们采用活体成像方法来观察蜗牛抑制因子，它确定了早期果蝇胚胎中预定中胚层和神经外胚层之间的边界。蜗牛靶增强子与一个MS2报告基因相连，从而能够检测活胚胎中的新生转录本。转基因最初呈现出广泛的转录模式，但在有丝分裂后中胚层中通过抑制作用而得到细化。这些观察结果揭示了有丝分裂沉默与蜗牛抑制之间的相关性。我们提出，有丝分裂和转录过程中的其他固有间断会增强序列特异性抑制因子（如蜗牛抑制因子）的活性。
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