Grenade Charlotte, De Pauw-Gillet Marie-Claire, Gailly Patrick, Vanheusden Alain, Mainjot Amélie
Dental Biomaterials Research Unit (d-BRU) and Department of Fixed Prosthodontics, Institute of Dentistry, University of Liège and University of Liège Hospital (ULg, CHU), Liège, Belgium.
Mammalian Cell Culture Laboratory - GIGA-R, University of Liège (ULg), Liège, Belgium.
Dent Mater. 2016 Sep;32(9):1152-64. doi: 10.1016/j.dental.2016.06.020. Epub 2016 Jul 8.
Polymer-infiltrated-ceramic-network (PICN) materials constitute an innovative class of CAD-CAM materials offering promising perspectives in prosthodontics, but no data are available in the literature regarding their biological properties. The objective of the present study was to evaluate the in vitro biocompatibility of PICNs with human gingival fibroblasts (HGFs) in comparison with materials typically used for implant prostheses and abutments.
HGF attachment, proliferation and spreading on discs made of PICN, grade V titanium (Ti), yttrium zirconia (Zi), lithium disilicate glass-ceramic (eM) and polytetrafluoroethylene (negative control), were evaluated using a specific insert-based culture system (IBS-R). Sample surface properties were characterized by XPS, contact angle measurement, profilometry and SEM.
Ti and Zi gave the best results regarding HGF viability, morphology, number and coverage increase with time in comparison with the negative control, while PICN and eM gave intermediate results, cell spreading being comparable for PICN, Ti, Zi and eM. Despite the presence of polymers and their related hydrophobicity, PICN exhibited comparable results to glass-ceramic materials, which could be explained by the mode of polymerization of the monomers.
The results of the present study confirm that the currently employed materials, i.e. Ti and Zi, can be considered to be the gold standard of materials in terms of HGF behavior, while PICN gave intermediate results comparable to eM. The impact of the present in vitro results needs to be further investigated clinically, particularly in the view of the utilization of PICNs for prostheses on bone-level implants.
聚合物渗透陶瓷网络（PICN）材料是一类创新的计算机辅助设计与制造（CAD-CAM）材料，在口腔修复学领域展现出广阔前景，但目前尚无关于其生物学特性的文献报道。本研究旨在评估PICN与人牙龈成纤维细胞（HGF）的体外生物相容性，并与常用于种植体修复体和基台的材料进行比较。
使用基于特定插入物的培养系统（IBS-R）评估HGF在由PICN、V级钛（Ti）、钇稳定氧化锆（Zi）、二硅酸锂玻璃陶瓷（eM）和聚四氟乙烯（阴性对照）制成的圆盘上的附着、增殖和铺展情况。通过X射线光电子能谱（XPS）、接触角测量、轮廓仪和扫描电子显微镜（SEM）对样品表面特性进行表征。
与阴性对照相比，Ti和Zi在HGF活力、形态、数量以及随时间的覆盖面积增加方面表现最佳，而PICN和eM表现居中，PICN、Ti、Zi和eM的细胞铺展情况相当。尽管存在聚合物及其相关的疏水性，但PICN与玻璃陶瓷材料的结果相当，这可以通过单体的聚合方式来解释。
本研究结果证实，就HGF行为而言，目前使用的材料即Ti和Zi可被视为材料的金标准，而PICN的结果居中，与eM相当。目前的体外研究结果在临床上的影响需要进一步研究，特别是考虑到PICN在骨水平种植体修复中的应用。
