Department of Prosthetic Dentistry, Faculty of Dentistry, Akdeniz University, 07058, Antalya, Turkey.
Department of Restorative Dentistry, Faculty of Dentistry, Akdeniz University, 07058, Antalya, Turkey.
Clin Oral Investig. 2021 Jan;25(1):329-336. doi: 10.1007/s00784-020-03377-3. Epub 2020 Jun 3.
The present study aimed to identify and quantify the elution of monomers of five different resin-based CAD/CAM blocks (RCBs) using HPLC.
Five different RCBs were used in the study: GC Cerasmart (CS), Voco Grandio blocs (GR), 3M Lava Ultimate (LU), Shofu Block (SB), and Vita Enamic (VE). Fifteen samples from each material were prepared using a low-speed precision diamond saw (ISOMET Buehler, Lake Bluff, IL, USA) at 5 × 5 × 4 mm size. After the preparation of samples, an extraction solution was mixed with %75/%25 ethanol/water. The samples were stored in the amber-colored bottles during three different immersed periods as 1 h, 24 h, and 90 days (n = 5). After immersion, 0.5 ml solutions were taken from each bottle and analyzed using HPLC.
A total of 16.7 μg/ml of monomers from SB, 13.4 μg/ml of monomers from GR, 13.2 μg/ml of monomers from CS, and 6.7 μg/ml of monomers from LU were found after 3-m immersion. TEGDMA after 3-m of immersion was only released from the SB group, and also BisEMA was released from the CS group. Among the specimens immersed for 1 h, UDMA was released the least from the LU group and the most from the GR group (p < 0.05). Correspondingly, 24 h and 3 m after immersion, the highest release of monomers was found in the GR (p < 0.05).
When the monomer release from RCBs was evaluated, it was shown that these materials released methacrylate-based monomers except VE, especially if they were kept in a solvent solution for a long time such as 3 m.
The novel resin-based CAD/CAM blocks might monomer release, which may cause cytotoxic effects. But, the detected amount of monomer release is below the estimated daily limit.
本研究旨在使用 HPLC 鉴定和量化五种不同的树脂基 CAD/CAM 块（RCB）的单体洗脱情况。
本研究使用了五种不同的 RCB：GC Cerasmart（CS）、Voco Grandio 块（GR）、3M Lava Ultimate（LU）、Shofu Block（SB）和 Vita Enamic（VE）。每种材料各制备 15 个样本，使用低速精密金刚石锯（美国伊利诺伊州莱克布鲁夫的 ISOMET Buehler）切割成 5×5×4mm 的尺寸。制备样本后，将提取溶液与 75%/25%乙醇/水混合。将样本储存在琥珀色的瓶子中，分别在 1 小时、24 小时和 90 天三个不同的浸泡期内浸泡（n=5）。浸泡后，从每个瓶子中取出 0.5ml 溶液，并使用 HPLC 进行分析。
在 3 个月的浸泡后，从 SB 中检测到 16.7μg/ml 的单体，从 GR 中检测到 13.4μg/ml 的单体，从 CS 中检测到 13.2μg/ml 的单体，从 LU 中检测到 6.7μg/ml 的单体。只有在 SB 组中检测到三甲基丙烯酸乙酯在 3 个月浸泡后的释放，而在 CS 组中也检测到了双甲基丙烯酸乙酯的释放。在浸泡 1 小时的样本中，从 LU 组中释放出的 UDMA 最少，从 GR 组中释放出的最多（p<0.05）。相应地，在浸泡 24 小时和 3 个月后，GR 中单体的释放量最高（p<0.05）。
当评估 RCB 中的单体释放情况时，发现这些材料除了 VE 之外，都释放出了甲基丙烯酸酯基单体，尤其是如果这些材料长时间浸泡在溶剂溶液中，如 3 个月。
新型树脂基 CAD/CAM 块可能会释放单体，这可能会导致细胞毒性作用。但是，检测到的单体释放量低于估计的日限量。
