一种在寡脱氧核苷酸5'末端引入巯基的简单方法。 - Suppr | 超能文献

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Characterization and sequence verification of thiolated deoxyoligonucleotides used for microarray construction.用于微阵列构建的硫醇化脱氧寡核苷酸的表征与序列验证

J Am Soc Mass Spectrom. 2006 Oct;17(10):1397-1400. doi: 10.1016/j.jasms.2006.07.011. Epub 2006 Aug 8.

2

Construction of oligonucleotide arrays on a glass surface using a heterobifunctional reagent, N-(2-trifluoroethanesulfonatoethyl)-N-(methyl)-triethoxysilylpropyl-3-amine (NTMTA).使用异双功能试剂N-(2-三氟乙磺酰基乙基)-N-(甲基)-三乙氧基甲硅烷基丙基-3-胺（NTMTA）在玻璃表面构建寡核苷酸阵列。

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3

本文引用的文献

1

A simple spectrophotometric determination of solid supported amino groups.一种简单的分光光度法测定固体负载氨基。

J Biochem Biophys Methods. 1986 Jun;12(5-6):349-54. doi: 10.1016/0165-022x(86)90072-2.

2

The synthesis of oligonucleotides containing a primary amino group at the 5'-terminus.5'-末端含有伯氨基的寡核苷酸的合成。

Nucleic Acids Res. 1987 Apr 10;15(7):3131-9. doi: 10.1093/nar/15.7.3131.

Optimized synthesis of phosphorothioate oligodeoxyribonucleotides substituted with a 5'-protected thiol function and a 3'-amino group.

5'-端带有保护硫醇功能基团且3'-端带有氨基的硫代磷酸酯寡脱氧核糖核苷酸的优化合成。

Nucleic Acids Res. 2000 Feb 1;28(3):818-25. doi: 10.1093/nar/28.3.818.

4

A general method for the synthesis of 3'-sulfhydryl and phosphate group containing oligonucleotides.一种合成含3'-巯基和磷酸基团寡核苷酸的通用方法。

Nucleic Acids Res. 1991 Jun 11;19(11):3019-25. doi: 10.1093/nar/19.11.3019.

5

A simple method for introducing a thiol group at the 5'-end of synthetic oligonucleotides.一种在合成寡核苷酸5'端引入硫醇基团的简单方法。

Nucleic Acids Res. 1991 Aug 25;19(16):4561. doi: 10.1093/nar/19.16.4561.

一种在寡脱氧核苷酸5'末端引入巯基的简单方法。 - Suppr | 超能文献

A simple method for the introduction of thiol group at 5'-termini of oligodeoxynucleotides.

作者信息

Gaur R K, Sharma P, Gupta K C

机构信息

DNA Synthesis Laboratory, CSIR Centre for Biochemicals, Delhi, India.

出版信息

Nucleic Acids Res. 1989 Jun 12;17(11):4404. doi: 10.1093/nar/17.11.4404.

DOI:10.1093/nar/17.11.4404

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC317974/

Abstract

摘要