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一种佛手柑提取物通过调节人脂肪组织间充质干细胞中的PPAR水平来减少脂肪生成并增加脂肪分解。

A Citrus bergamia Extract Decreases Adipogenesis and Increases Lipolysis by Modulating PPAR Levels in Mesenchymal Stem Cells from Human Adipose Tissue.

作者信息

Lo Furno Debora, Graziano Adriana Carol Eleonora, Avola Rosanna, Giuffrida Rosario, Perciavalle Vincenzo, Bonina Francesco, Mannino Giuliana, Cardile Venera

机构信息

Department of Biomedical and Biotechnological Sciences, Section of Physiology, University of Catania, Via S. Sofia 65, 95125 Catania, Italy.

Department of Drug Sciences, University of Catania, Viale A. Doria 6, 95125 Catania, Italy.

出版信息

PPAR Res. 2016;2016:4563815. doi: 10.1155/2016/4563815. Epub 2016 Jun 15.

DOI:10.1155/2016/4563815
PMID:27403151
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4926019/
Abstract

The aim of this research was to assess the impact of a well-characterized extract from Citrus bergamia juice on adipogenesis and/or lipolysis using mesenchymal stem cells from human adipose tissue as a cell model. To evaluate the effects on adipogenesis, some cell cultures were treated with adipogenic medium plus 10 or 100 μg/mL of extract. To determine the properties on lipolysis, additional mesenchymal stem cells were cultured with adipogenic medium for 14 days and after this time added with Citrus bergamia for further 14 days. To verify adipogenic differentiation, oil red O staining at 7, 14, 21, and 28 days was performed. Moreover, the expression of peroxisome proliferator-activated receptor gamma (PPAR-γ), adipocytes fatty acid-binding protein (A-FABP), adipose triglyceride lipase (ATGL), hormone-sensitive lipase (HSL), monoglyceride lipase (MGL), 5'-adenosine monophosphate-activated protein kinase (AMPK)α1/2, and pAMPKα1/2 was evaluated by Western blot analysis and the release of glycerol by colorimetric assay. Citrus bergamia extract suppressed the accumulation of intracellular lipids in mesenchymal stem cells during adipogenic differentiation and promoted lipolysis by repressing the expression of adipogenic genes and activating lipolytic genes. Citrus bergamia extract could be a useful natural product for improving adipose mobilization in obesity-related disorders.

摘要

本研究的目的是使用来自人类脂肪组织的间充质干细胞作为细胞模型，评估特征明确的香柠檬果汁提取物对脂肪生成和/或脂肪分解的影响。为了评估对脂肪生成的影响，一些细胞培养物用脂肪生成培养基加10或100μg/mL提取物处理。为了确定对脂肪分解的作用，将另外的间充质干细胞用脂肪生成培养基培养14天，此后再添加香柠檬14天。为了验证脂肪生成分化，在第7、14、21和28天进行油红O染色。此外，通过蛋白质印迹分析评估过氧化物酶体增殖物激活受体γ（PPAR-γ）、脂肪细胞脂肪酸结合蛋白（A-FABP）、脂肪甘油三酯脂肪酶（ATGL）、激素敏感性脂肪酶（HSL）、单酰甘油脂肪酶（MGL）、5'-腺苷单磷酸激活蛋白激酶（AMPK）α1/2和pAMPKα1/2的表达，并通过比色法测定甘油的释放。香柠檬提取物在脂肪生成分化过程中抑制间充质干细胞内脂质的积累，并通过抑制脂肪生成基因表达和激活脂肪分解基因促进脂肪分解。香柠檬提取物可能是一种用于改善肥胖相关疾病中脂肪动员的有用天然产物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6624/4926019/80591a7a753b/PPAR2016-4563815.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6624/4926019/4324e119f214/PPAR2016-4563815.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6624/4926019/723de8493cb2/PPAR2016-4563815.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6624/4926019/a2d19607939d/PPAR2016-4563815.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6624/4926019/a7cdbcad573a/PPAR2016-4563815.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6624/4926019/d152c47cb4bd/PPAR2016-4563815.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6624/4926019/80591a7a753b/PPAR2016-4563815.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6624/4926019/4324e119f214/PPAR2016-4563815.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6624/4926019/723de8493cb2/PPAR2016-4563815.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6624/4926019/a2d19607939d/PPAR2016-4563815.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6624/4926019/a7cdbcad573a/PPAR2016-4563815.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6624/4926019/d152c47cb4bd/PPAR2016-4563815.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6624/4926019/80591a7a753b/PPAR2016-4563815.006.jpg

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