College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400716, China ; Key Laboratory of Horticulture Science for Southern Mountainous Regions, Ministry of Education, Chongqing 400715, China ; School of Pharmacy, Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Shanghai 201203, China.
Evid Based Complement Alternat Med. 2012;2012:678592. doi: 10.1155/2012/678592. Epub 2012 Dec 19.
Obesity is a common nutritional disorder associated with type 2 diabetes, cardiovascular diseases, dyslipidemia, and certain cancers. In this study, we investigated the effects of Citrus ichangensis peel extract (CIE) in high-fat (HF) diet-induced obesity mice. Female C57BL/6 mice were fed a chow diet or an HF diet alone or supplemented with 1% w/w CIE for 8 weeks. We found that CIE treatment could lower blood glucose level and improve glucose tolerance. In the HF+CIE group, body weight gain, serum total cholesterol (TC) and low-density lipoprotein cholesterol (LDL-c) levels, and liver triglyceride (TG) and TC concentrations were significantly (P < 0.05) decreased relative to those in the HF group. To elucidate the mechanism of CIE on the metabolism of glucose and lipid, related genes expression in liver were examined. In liver tissue, CIE significantly decreased the mRNA expression levels of peroxisome proliferator-activated receptor γ (PPARγ) and its target genes, such as fatty acid synthase (FAS) and acyl-CoA oxidase (ACO). Moreover, CIE also decreased the expression of liver X receptor (LXR) α and β which are involved in lipid and glucose metabolism. These results suggest that CIE administration could alleviate obesity and related metabolic disorders in HF diet-induced obesity mice through the inhibition of PPARγ and LXR signaling.
肥胖是一种常见的营养障碍，与 2 型糖尿病、心血管疾病、血脂异常和某些癌症有关。在这项研究中，我们研究了宜昌橙皮提取物（CIE）对高脂肪（HF）饮食诱导肥胖小鼠的作用。雌性 C57BL/6 小鼠喂食标准饮食或 HF 饮食，或补充 1%w/w 的 CIE 共 8 周。结果发现，CIE 处理可降低血糖水平并改善葡萄糖耐量。在 HF+CIE 组中，与 HF 组相比，体重增加、血清总胆固醇（TC）和低密度脂蛋白胆固醇（LDL-c）水平以及肝甘油三酯（TG）和 TC 浓度显著（P<0.05）降低。为了阐明 CIE 对葡萄糖和脂质代谢的作用机制，我们检测了肝脏中相关基因的表达。在肝组织中，CIE 显著降低了过氧化物酶体增殖物激活受体γ（PPARγ）及其靶基因，如脂肪酸合酶（FAS）和酰基辅酶 A 氧化酶（ACO）的 mRNA 表达水平。此外，CIE 还降低了参与脂质和葡萄糖代谢的肝 X 受体（LXR）α和β的表达。这些结果表明，CIE 给药可通过抑制 PPARγ 和 LXR 信号通路缓解 HF 饮食诱导肥胖小鼠的肥胖和相关代谢紊乱。
