Matseke Buang, Mapfumari Sipho, Mothibe Mmamosheledi
Department of Pharmaceutical Sciences, School of Pharmacy, Sefako Makgatho Health Sciences University, P.O. Box 218, Pretoria 0208, South Africa.
Department of Physiology, School of Medicine, Sefako Makgatho Health Sciences University, P.O. Box 211, Pretoria 0208, South Africa.
Life (Basel). 2024 Dec 24;15(1):4. doi: 10.3390/life15010004.
Linn is a well-known African traditional herb due to its tremendous medicinal and nutritional properties. It is used worldwide for the treatment of different ailments and diseases. In the present study, the phytochemical and antioxidant activity of South African fruit pulp extracts was evaluated. The fruit pulp extracts were obtained by using the serial exhaustive extraction procedure using the solvents hexane, DCM, acetone, and methanol. The resulting extracts were subjected to different standard colorimetric tests for phytochemical analysis. The presence of compounds with antioxidant activity was determined using dot plot and TLC. The DPPH radical scavenging assay, hydrogen peroxide activity, and reducing power assay, coupled with linear regression, were employed to determine the quantity of antioxidants and their IC50. The results for qualitative phytochemical screening have shown that the fruit pulp contains alkaloids, cardiac glycosides, saponins, phenolic compounds, tannins, flavonoids, terpenoids, and steroids. All the extracts revealed the presence of antioxidant activity in both dot plot and TLC. Acetone extracts (0.279 mg/mL) showed the lowest IC50 compared to the standards gallic acid (0.4 mg/mL) and diosgenin (0.42 mg/mL). These findings confirmed that is very rich in phytochemical compounds and has strong antioxidant potential; therefore, it could be a potential source of drugs which in the future may serve the production of synthetically improved therapeutic agents.
由于其巨大的药用和营养特性，Linn是一种著名的非洲传统草药。它在全球范围内被用于治疗各种疾病。在本研究中，对南非水果果肉提取物的植物化学成分和抗氧化活性进行了评估。通过使用己烷、二氯甲烷、丙酮和甲醇等溶剂的连续 exhaustive提取程序获得水果果肉提取物。对所得提取物进行不同的标准比色试验以进行植物化学分析。使用点图和薄层色谱法测定具有抗氧化活性的化合物的存在。采用DPPH自由基清除试验、过氧化氢活性试验和还原能力试验，并结合线性回归，来确定抗氧化剂的含量及其IC50。定性植物化学筛选结果表明，水果果肉含有生物碱、强心苷、皂苷、酚类化合物、单宁、黄酮类化合物、萜类化合物和甾体。所有提取物在点图和薄层色谱法中均显示出抗氧化活性。与标准品没食子酸（0.4 mg/mL）和薯蓣皂苷元（0.42 mg/mL）相比，丙酮提取物（0.279 mg/mL）显示出最低的IC50。这些发现证实，[原文此处Linn未翻译完整，疑似有误]富含植物化学化合物且具有很强的抗氧化潜力；因此，它可能是一种潜在的药物来源，未来可能用于生产合成改良的治疗剂。
