Banik Debasis, Dutta Rupam, Banerjee Pavel, Kundu Sangita, Sarkar Nilmoni
Department of Chemistry, Indian Institute of Technology , Kharagpur 721302, WB, India.
J Phys Chem B. 2016 Aug 11;120(31):7662-70. doi: 10.1021/acs.jpcb.6b05209. Epub 2016 Jul 27.
In this article, our aim is to investigate the interaction of l-phenylalanine (l-Phe) fibrils with crown ethers (CEs). For this purpose, two different CEs (15-Crown-5 (15C5) and 18-Crown-6 (18C6)) were used. Interestingly, we have observed that both CEs have the ability to arrest fibril formation. However, 18C6 was found to be a better candidate compared to 15C5. Field emission scanning electron microscopy and fluorescence lifetime imaging microscopy were used to monitor the fibril-arresting kinetics of CEs. The arresting process was further confirmed by fluorescence correlation spectroscopy and nuclear magnetic resonance studies.
在本文中，我们的目的是研究L-苯丙氨酸（L-Phe）纤维与冠醚（CEs）之间的相互作用。为此，使用了两种不同的冠醚（15-冠-5（15C5）和18-冠-6（18C6））。有趣的是，我们观察到两种冠醚都有阻止纤维形成的能力。然而，发现18C6比15C5更适合。利用场发射扫描电子显微镜和荧光寿命成像显微镜来监测冠醚的纤维阻止动力学。通过荧光相关光谱和核磁共振研究进一步证实了阻止过程。
