Schulte Marc, Hensel Michael
Department of Microbiology, University of Osnabrück, Osnabrück, Germany.
F1000Res. 2016 Jun 24;5. doi: 10.12688/f1000research.8468.1. eCollection 2016.
Salmonella enterica serovar Typhimurium is a foodborne pathogen causing inflammatory disease in the intestine following diarrhea and is responsible for thousands of deaths worldwide. Many in vitro investigations using cell culture models are available, but these do not represent the real natural environment present in the intestine of infected hosts. Several in vivo animal models have been used to study the host-pathogen interaction and to unravel the immune responses and cellular processes occurring during infection. An animal model for Salmonella-induced intestinal inflammation relies on the pretreatment of mice with streptomycin. This model is of great importance but still shows limitations to investigate the host-pathogen interaction in the small intestine in vivo. Here, we review the use of mouse models for Salmonella infections and focus on a new small animal model using 1-day-old neonate mice. The neonate model enables researchers to observe infection of both the small and large intestine, thereby offering perspectives for new experimental approaches, as well as to analyze the Salmonella-enterocyte interaction in the small intestine in vivo.
肠炎沙门氏菌鼠伤寒血清型是一种食源性病原体，在腹泻后会引发肠道炎症性疾病，在全球范围内导致数千人死亡。目前有许多使用细胞培养模型的体外研究，但这些模型并不能代表受感染宿主肠道中的真实自然环境。已经使用了几种体内动物模型来研究宿主与病原体的相互作用，并揭示感染期间发生的免疫反应和细胞过程。一种用于沙门氏菌诱导的肠道炎症的动物模型依赖于用链霉素对小鼠进行预处理。该模型非常重要，但在研究体内小肠中的宿主与病原体相互作用方面仍存在局限性。在这里，我们回顾了小鼠模型在沙门氏菌感染研究中的应用，并重点介绍了一种使用1日龄新生小鼠的新型小动物模型。新生小鼠模型使研究人员能够观察小肠和大肠的感染情况，从而为新的实验方法提供了前景，也能够在体内分析小肠中沙门氏菌与肠上皮细胞的相互作用。
