Davis Destiny J, McDowell Stephen C, Park Eunsook, Hicks Glenn, Wilkop Thomas E, Drakakaki Georgia
Plant Signal Behav. 2016;11(3):e984520. doi: 10.4161/15592324.2014.984520.
Cytokinesis in plants requires the activity of RAB GTPases to regulate vesicle-mediated contribution of material to the developing cell plate. While some plant RAB GTPases have been shown to be involved in cell plate formation, many still await functional assignment. Here, we report cell plate localization for YFP-RABA1e in Arabidopsis thaliana and use the cytokinesis inhibitor Endosidin 7 to provide a detailed description of its localization compared to YFP-RABA2a. Differences between YFP-RABA2a and YFP-RABA1e were observed in late-stage cell plates under DMSO control treatment, and became more apparent under Endosidin 7 treatment. Taken together, our results suggest that individual RAB GTPases might make different contributions to cell plate formation and further demonstrates the utility of ES7 probe to dissect them.
植物中的胞质分裂需要RAB GTP酶的活性来调节囊泡介导的物质对发育中细胞板的贡献。虽然一些植物RAB GTP酶已被证明参与细胞板形成，但许多仍有待功能鉴定。在这里，我们报告了拟南芥中YFP-RABA1e的细胞板定位，并使用胞质分裂抑制剂Endosidin 7与YFP-RABA2a相比详细描述其定位。在二甲基亚砜对照处理下的后期细胞板中观察到YFP-RABA2a和YFP-RABA1e之间的差异，在Endosidin 7处理下变得更加明显。总之，我们的结果表明，单个RAB GTP酶可能对细胞板形成有不同贡献，并进一步证明了ES
