Department of Microbiology, Soochow University, Taipei, Taiwan.
Environ Sci Pollut Res Int. 2016 Oct;23(19):19754-63. doi: 10.1007/s11356-016-7187-y. Epub 2016 Jul 13.
This study investigated the degradation of sulfonamide antibiotics (SAs) and microbial community changes in sludge-amended soil. In batch experiments, SA degradation was enhanced by addition of spent mushroom compost (SMC), SMC extract, and extract-containing microcapsule, with SMC showing higher SA degradation rate than the other additives in soil-sludge mixtures. In bioreactor experiments, the degradation of SAs in soil-sludge mixtures was in the order of sulfamethoxazole > sulfadimethoxine > sulfamethazine during four times of SA addition. SA removal was higher in soil-sludge mixtures than in soil alone. The bacterial composition differed in soil-sludge mixtures with and without SMC. In total, 44 differentially distributed bacterial genera were identified from different experimental settings and stages. Four bacterial genera, Acinetobacter, Alcaligenes, Brevundimonas, and Pseudomonas, were previously found involved in SA degradation, and 20 of the 44 bacterial genera were previously found in aromatic hydrocarbon degradation. Therefore, these bacteria have high potential to be SA degradation bacteria in this study.
本研究考察了在添加污泥的土壤中，磺胺类抗生素（SAs）的降解和微生物群落变化。在批处理实验中，通过添加废蘑菇堆肥（SMC）、SMC 提取物和含提取物的微胶囊，增强了 SAs 的降解，SMC 在土壤-污泥混合物中表现出比其他添加剂更高的 SAs 降解速率。在生物反应器实验中，在四次添加 SAs 期间，土壤-污泥混合物中 SAs 的降解顺序为磺胺甲恶唑>磺胺二甲氧嘧啶>磺胺甲嗪。在土壤-污泥混合物中，SAs 的去除率高于单独的土壤。有和没有 SMC 的土壤-污泥混合物中的细菌组成不同。在总共 44 个不同的实验设置和阶段中，鉴定出了 44 个差异分布的细菌属。先前发现四种细菌属（不动杆菌属、产碱杆菌属、短小杆菌属和假单胞菌属）参与了 SAs 的降解，而这 44 个细菌属中有 20 个先前在芳烃降解中发现。因此，这些细菌在本研究中极有可能成为 SAs 的降解菌。
