Uzêda-E-Silva Virgínia Dias, Rodriguez Tania Tavares, Rocha Isadora Almeida Rios, Xavier Flávia Calo Aquino, Dos Santos Jean Nunes, Cury Patrícia Ramos, Ramalho Luciana Maria Pedreira
School of Dentistry, Federal University of Bahia, Avenida Araújo Pinho, 62. Canela, Salvador, BA, CEP 40110-150, Brazil.
Department of Physiology, Health Sciences Center, Federal University of Bahia, Salvador, Brazil.
Lasers Med Sci. 2016 Sep;31(7):1363-70. doi: 10.1007/s10103-016-1985-9. Epub 2016 Jul 13.
The aim of this study was to evaluate the influence of laser photobiomodulation in cutaneous healing of rats under a hyperlipidic diet. Forty-eight Wistar Albinus rats, weaned, received standard diet (SD) or hyperlipidic diet (HD) for 20 weeks. The groups were divided into SD rats and HD rats, SD-irradiated rats (LSD), and HD-irradiated rats (LHD). Standard cutaneous wound (1 cm(2)) was created on the dorsum of each rat. The irradiation started immediately after surgery and every 48 h for 7 or 14 days (λ660 nm, 40 mW, 6 J/cm(2), ϕ 0,04 cm(2), CW), when they were killed under deep anesthesia. The specimens were removed, routinely processed, stained with hematoxylin/eosin (H/E), and evaluated by light microscopy. Rats fed with hyperlipidic diet had greater intensity in the inflammatory process and prolonged hyperemia. At day 7, the intensity of inflammation was reduced in LSD and LHD groups when compared to their control groups, SD (p = 0.002) and HD (p = 0.02). There was an increase in fibroblast proliferation and collagen deposition, especially in the LHD group. At day 14, the HD group presented more intensive hyperemia than the SD group. It can be concluded that the hyperlipidic diet modified the inflammation pattern in wound healing and that laser light has a positive biomodulative effect on the healing process only in early stages.
本研究的目的是评估激光光生物调节对高脂饮食大鼠皮肤愈合的影响。48只断奶的Wistar白化大鼠接受标准饮食（SD）或高脂饮食（HD）20周。这些组被分为SD大鼠和HD大鼠、SD照射大鼠（LSD）和HD照射大鼠（LHD）。在每只大鼠的背部制造标准皮肤伤口（1平方厘米）。手术后立即开始照射，每48小时照射一次，持续7天或14天（波长660纳米，40毫瓦，6焦/平方厘米，光斑直径0.04厘米，连续波），之后在深度麻醉下将它们处死。取出标本，常规处理，用苏木精/伊红（H/E）染色，并通过光学显微镜进行评估。高脂饮食喂养的大鼠炎症过程强度更大，充血时间延长。在第7天，与对照组SD（p = 0.002）和HD（p = 0.02）相比，LSD和LHD组的炎症强度降低。成纤维细胞增殖和胶原蛋白沉积增加，尤其是在LHD组。在第14天，HD组的充血比SD组更强烈。可以得出结论，高脂饮食改变了伤口愈合中的炎症模式，并且激光仅在早期对愈合过程具有积极的生物调节作用。
