Center of Biophotonics, School of Dentistry, Federal University of Bahia, Av. Araújo Pinho, 62, Canela, Salvador, Bahia, CEP 40110-150, Brazil,
Lasers Med Sci. 2014 Jul;29(4):1405-10. doi: 10.1007/s10103-014-1537-0. Epub 2014 Feb 20.
Laser and LED phototherapies accelerate tissue repair. Mast cells induce the proliferation of fibroblasts and the development of local fibrosis. Increased numbers of myofibroblasts and mast cells are frequently found together in a normal wound repair, suggesting that mediators produced by the mast cells could play a role in the regulation of myofibroblast differentiation and function. The aim of this study was to analyze the involvement of mast cells on the synthesis of collagen and their influence on myofibroblast differentiation in the late phase of tissue repair on wounds treated with LLLT (λ 660 nm, 10 J/cm(2), 40 mW, 252 s) or LED (λ 630 ± 10 nm, 10 J/cm(2), 115 mW, 87 s). A 1 × 1-cm surgical wound was created on the dorsum of 30 rats divided into three groups of ten animals each: control, laser, and LED. The animals of each group were irradiated and sacrificed 7 and 14 days after injury. The statistical analysis was performed using the Mann-Whitney and Spearman correlation tests. Laser light improved the collagen deposition rate along the time points (p = 0.22), but when compared to the control groups during the periods studied, the number of mast cells decreased significantly (p ≤ 0.05). With respect to myofibroblasts, the results showed a trend to their reduction. No statistical significances were observed for LED light according to the parameters used in this study. It is concluded that the mast cell and myofibroblast population might participate in the collagen formation of irradiated wounds particularly in relation to laser phototherapy.
激光和 LED 光疗可加速组织修复。肥大细胞可诱导成纤维细胞增殖和局部纤维化形成。在正常伤口修复中，常常可以发现肌成纤维细胞和肥大细胞数量增加，这表明肥大细胞产生的介质可能在调节肌成纤维细胞分化和功能中发挥作用。本研究旨在分析肥大细胞在激光光疗（λ 660nm，10J/cm²，40mW，252s）或 LED（λ 630±10nm，10J/cm²，115mW，87s）治疗的伤口组织修复晚期胶原合成中的作用及其对肌成纤维细胞分化的影响。在 30 只大鼠背部创建了一个 1×1cm 的手术伤口，将它们分为三组，每组 10 只动物：对照组、激光组和 LED 组。每组动物在受伤后 7 和 14 天进行照射和处死。使用 Mann-Whitney 和 Spearman 相关检验进行统计分析。激光光疗可改善胶原沉积率随时间的变化（p=0.22），但与对照组相比，在研究期间，肥大细胞数量显著减少（p≤0.05）。关于肌成纤维细胞，结果显示其数量有减少的趋势。根据本研究中使用的参数，LED 光没有观察到统计学意义。结论是，肥大细胞和肌成纤维细胞可能参与了照射伤口的胶原形成，特别是与激光光疗有关。
