Rozanov V A, Reĭtarova T E
Ukr Biokhim Zh (1978). 1989 Jan-Feb;61(1):42-8.
The comparison has been made for the following items: intensity of pyruvate alpha-ketoglutarate, succinate oxidation, the gamma-aminobutyric acid (GABA) formation rate, utilization, total content of GABA, glutamate and alanine, the bound/free form ratio of GABA and glutamate, intensity of binding and desorption of exogenic [I-14C]GABA in homogenates of the cortex, cerebellum and brainstem of the Wistar rats. It is revealed that the intensity of ketoacids oxidation is significantly lower in the cerebellum than in the cortex, but the maximal activity of the enzymes of GABA formation and utilization is higher, due to which considerable oxidation of alpha-ketoglutarate transforming into succinate is possible proceeding the GABA shunt pathway. The cortex homogenates contrary to the cerebellum ones are characterized by the reliably higher intensity of ketoacid oxidation and by insignificant contribution of the GABA-shunt to the succinate production. These differences are in line with the reliably higher content of endogenic bound GABA in the cortex as compared to the cerebellum, with a higher level of binding of exogenic labelled GABA and with less pronounced desorption of the label from neurostructures. An assumption is advanced that the observed differences are related to the known high sensitivity of the cortex and to relative resistance of cerebellum to hypoxia and hypoglycemia.
丙酮酸α-酮戊二酸的强度、琥珀酸氧化、γ-氨基丁酸（GABA）的生成速率、利用率、GABA、谷氨酸和丙氨酸的总含量、GABA和谷氨酸的结合/游离形式比率、外源性[I-14C]GABA在Wistar大鼠皮层、小脑和脑干匀浆中的结合和解吸强度。结果显示，小脑内酮酸氧化强度显著低于皮层，但GABA生成和利用酶的最大活性较高，这使得通过GABA分流途径，α-酮戊二酸大量氧化转化为琥珀酸成为可能。与小脑匀浆相反，皮层匀浆的特点是酮酸氧化强度确实较高，且GABA分流对琥珀酸生成的贡献微不足道。这些差异与皮层中内源性结合GABA的含量确实高于小脑、外源性标记GABA的结合水平较高以及神经结构中标记物的解吸不太明显一致。有人提出一种假设，即观察到的差异与已知的皮层高敏感性以及小脑对缺氧和低血糖的相对抗性有关。
